Vet World Vol.13 February-2020 Article-5
Research Article
Veterinary World, 13(2): 256-260
https://doi.org/10.14202/vetworld.2020.256-260
Inflammation in the lungs of mice due to methyl methacrylate exposure
2. Department of Pathology Anatomy, Faculty of Medicine, Universitas Airlangga, Surabaya 60132, East Java, Indonesia.
3. Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, East Java, Indonesia.
Background and Aim: This study aimed to predict the potential inflammation in lungs caused by exposure to methyl methacrylate (MMA; in silico study) and assess inflammation in lungs in response to MMA inhalation in mice (in vivo study).
Materials and Methods: In silico and in vivo studies were performed using 24 mice divided into a control group (0 ppm MMA) and five treatment groups, which were exposed to 150 ppm MMA for 40, 80, 120, 160, and 200 min, respectively. Lung tissues were harvested and examined with a light microscope at 400×.
Results: In silico studies confirmed the existence of one activation bond between MMA and the toll-like receptor 4 (TLR- 4), namely, His 228, with a MolDock score of –43.677 kcal/mol. Microscopic examination of lungs confirmed that a greater number of inflammatory cells were found in the treatment group than in the control group and symptoms of inflammation were clearly observable after 120 min of exposure.
Conclusion: Thus, inflammation occurring due to MMA interaction with TLR-4 receptors can be predicted in silico and exposure to 150 ppm MMA for more than 120 min can cause lung inflammation in mice. Keywords: inflammation, inhalation, in silico, methyl methacrylate.
Keywords: inflammation, inhalation, in silico, methyl methacrylate.
How to cite this article: Goenharto S, Sudiana IK, Salim S, Rusdiana E, Wahjuni S (2020) Inflammation in the lungs of mice due to methyl methacrylate exposure, Veterinary World, 13(2): 256-260.
Received: 14-10-2019 Accepted: 30-12-2019 Published online: 11-02-2020
Corresponding author: Sianiwati Goenharto E-mail: sianiwati-g@fkg.unair.ac.id
DOI: 10.14202/vetworld.2020.256-260
Copyright: Goenharto, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.