Vet World   Vol.13   January-2020  Article-19

Research Article

Veterinary World, 13(1): 127-133

https://doi.org/10.14202/vetworld.2020.127-133

Effect of insulin-like growth factor 1 gene on growth traits of Kejobong goat and its growth analysis

Dela Ayu Lestari1, Takuro Oikawa2, Sutopo Sutopo1, Endang Purbowati1, Asep Setiaji1,2,3, and Edy Kurnianto1
1. Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang, Central Java 50275, Indonesia.
2. Department of Subtropical Agro-Environmental Sciences, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan.
3. United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima 890-8580, Japan.

Background and Aim: This study aimed to identify the effect of the insulin-like growth factor 1 (IGF1) gene on growth, to uncover the genetic marker at the IGF1 gene, and to predict growth performance by analyzing growth models of Kejobong goats based on their genotype.

Materials and Methods: DNA and records of body weight (BW) and body measurements (BM) of Kejobong goats were collected, the IGF1 gene was amplified from the DNA template by polymerase chain reaction (PCR); the PCR products were then sequenced to determine single nucleotide polymorphisms (SNP). Linear mixed model (LMM) was used to analyze the association between SNP and growth traits. Four non-linear growth models were analyzed using non-LMM to describe the growth model and to compare the growth within genotypes.

Results: An SNP at intron 4 (g5752G→C) genotyped into GG and CC was significantly associated with BW and BM. Goats of genotype GG had a significantly higher BW and BM (p<0.05) than those of genotype CC. Growth analysis showed that the von Bertalanffy model was the most fit for describing BW, the Brody model for chest width and hip height, the Gompertz and Logistic models for heart girth, and the von Bertalanffy and Gompertz models for hip width.

Conclusion: An SNP at intron 4 of the IGF1 gene was associated with the growth trait and was usable as a genetic marker candidate for improvement of growth traits of Kejobong goats while von Bertalanffy model provides proper and accurate estimates of parameters to describe the growth performance of Kejobong goats. Keywords: genetic markers, goat, growth analysis, growth traits, insulin-like growth factor 1.

Keywords: genetic markers, goat, growth analysis, growth traits, insulin-like growth factor 1.

How to cite this article: Lestari DA, Oikawa T, Sutopo S, Purbowati E, Setiaji A, Kurnianto E (2020) Effect of insulin-like growth factor 1 gene on growth traits of Kejobong goat and its growth analysis, Veterinary World, 13(1): 127-133.

Received: 07-09-2019  Accepted: 09-12-2019     Published online: 18-01-2020

Corresponding author: Edy Kurnianto   E-mail: kurniantoedy17@gmail.com

DOI: 10.14202/vetworld.2020.127-133

Copyright: Lestari, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.