Structure of cytotoxic associated antigen A protein of Helicobacter pylori from Bali and Lombok isolates of Indonesia

Vet World Vol.13 July-2020 Article-10

Research Article

Veterinary World, 13(7): 1319-1326

https://doi.org/10.14202/vetworld.2020.1319-1326

Hamong Suharsono¹, Dewa Nyoman Wibawa², Zainul Muttaqin³, and Kadek Karang Agustina⁴

1. Laboratory of Biochemistry, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.
2. Internal Medicine Laboratory, Faculty of Medicine Udayana University, Denpasar, Indonesia.
3. Biomedical Research Unit, West Nusa Tenggara General Hospital, Lombok, Indonesia.
4. Department of Veterinary Public Health, Faculty of Veterinary Medicine Udayana University, Denpasar, Indonesia.

Background and Aim: Helicobacter pylori is a well-known zoonotic agent with worldwide distribution. In Indonesia, only one report regarding the variation within the cytotoxic associated antigen A (CagA) protein of H. pylori has been described in the literature, which was conducted in Manado, South Sulawesi. There remains no report concerning the structure of this protein, particularly for the Bali and Lombok isolates. The objective of this study was to investigate the diversity of H. pylori CagA amino acid sequences of Bali and Lombok isolates, to predict their molecular structures and conduct toxicity examination of CagA on gastric cells.

Materials and Methods: A total of 36 samples were used in equal proportions for each pathologic condition. DNA extraction was performed to subculture H. pylori Bali isolates. The amplification of the CagA 3' variable region was carried out using the primers P1 (5'-GATAACAGGCAAGCTTTTTGAGG-3') and P2 (5'-CTGCAAAAGATTGTTTGGCAG-3'). The W2, W9, and W35 fragments were selected as a representation of H. pylori Bali isolates, which were modeled through the threading modeling approach using I-TASSER.

Results: According to the 12 CagA sequences obtained and phylogenetic analyses, the H. pylori strain originating from Bali can be grouped within the East Asian genotypes and is identical to the Lombok strain. In addition, the Bali isolates are phylogenetically more closely related to Southeast Asian strains, particularly the Filipino strain. The relationship between degree of inflammation induced and CagA-positive infection was not statistically significant.

Conclusion: The structure of the H. pylori Bali isolate is identical to that of Lombok isolate, which belongs to the same group of East Asian genotypes, and bacterial virulence is not related to structure. Keywords: Bali, cytotoxic associated antigen A, Helicobacter pylori, Lombok, structure, virulence.

Keywords: Bali, cytotoxic associated antigen A, Helicobacter pylori, Lombok, structure, virulence.

How to cite this article: Suharsono H, Wibawa DN, Muttaqin Z, Agustina KK (2020) Structure of cytotoxic associated antigen A protein of Helicobacter pylori from Bali and Lombok isolates of Indonesia, Veterinary World, 13(7): 1319-1326.

Received: 31-12-2019 Accepted: 21-05-2020 Published online: 13-07-2020

Corresponding author: Hamong Suharsono E-mail: hamong@unud.ac.id

DOI: 10.14202/vetworld.2020.1319-1326

Copyright: Suharsono, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.