Vet World Vol.13 July-2020 Article-12
Research Article
Veterinary World, 13(7): 1334-1343
https://doi.org/10.14202/vetworld.2020.1334-1343
Effects of polymorphism of myostatin and fatty acid-binding protein 4 genes on the chemical composition of meat in cull female Aceh cattle
2. Department of Histology , Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh 23111, Indonesia.
3. Department of Parasitology , Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh 23111, Indonesia.
4. Department of Anatomy , Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh 23111, Indonesia.
5. Master Program of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh 23111, Indonesia.
Background and Aim: This study aimed to investigate the association of single nucleotide polymorphism of the myostatin (MSTN) and fatty acid-binding protein 4 (FABP4) genes on the total water, ash, fat, protein, and cholesterol contents of sirloin (gluteus medius muscle) and silverside (biceps femoris muscle) meats of cull female Aceh cattle.
Materials and Methods: This analysis covered a total of 27 cull female Aceh cattle slaughtered at the Animal Slaughterhouse of Banda Aceh that was purposively selected based on hair color referred to the criteria described in the Decree of Ministry of Agriculture of the Republic of Indonesia. Genomic DNA was extracted from 25 mg of fresh meat using the spin column method before subjected to a polymerase chain reaction amplification using primer sets specific for 1346-bp and 275- bp fragments of MSTN and FABP4, respectively. A 4-h digestion reaction was done separately for the MSTN/HaeIII and FABP4/NlaIII loci genotyping. The total protein, ash, and fat of the meat were measured using the Indonesian National Standard (SNI) methods whereas its cholesterol content was determined using the AOAC method. The association between each polymorphism and the variation in meat chemical parameters was analyzed using the Pearson correlation test.
Results: The results showed that the MSTN/HaeIII locus was polymorphic in Aceh cattle, but the FABP4/NlaIII locus was monomorphic. Meat chemical parameters were not influenced by different commercial cuts and MSTN genotypes, showing that there was no association between different commercial cuts, cattle hair colors, and MSTN/HaeIII and FABP4/NlaIII markers with the meat chemical parameters in Aceh cattle.
Conclusion: These results suggest that focusing on the novel effects of MSTN and FABP4 gene polymorphisms on meat production traits might not be useful for marker-assisted selection in Aceh cattle. Keywords: Aceh cattle meat, ash, cholesterol, fat, polymerase chain reaction-restriction fragment length polymorphism, protein.
Keywords: Aceh cattle meat, ash, cholesterol, fat, polymerase chain reaction-restriction fragment length polymorphism, protein.
How to cite this article: Azhar A, Akmal M, Hambal M, Sabri M, Rosa TS (2020) Effects of polymorphism of myostatin and fatty acid-binding protein 4 genes on the chemical composition of meat in cull female Aceh cattle, Veterinary World, 13(7): 1334-1343.
Received: 18-08-2019 Accepted: 14-05-2020 Published online: 14-07-2020
Corresponding author: Al Azhar E-mail: alazhar@unsyiah.ac.id
DOI: 10.14202/vetworld.2020.1334-1343
Copyright: Azhar, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.