Vet World Vol.13 July-2020 Article-21
Research Article
Veterinary World, 13(7): 1404-1409
https://doi.org/10.14202/vetworld.2020.1404-1409
Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice
2. Department of Microbiology, Faculty of Medicine and Health Sciences, State Islamic University of Maulana Malik Ibrahim Malang, Malang 65144, East Java, Indonesia.
Background and Aim: Hyperlipidemia is an important risk factor for cardiovascular disease. The use of statins has adverse side effects that result in oxidative stress disorders. The objective of this study was to investigate the antihyperlipidemic effect of a combination of Cinnamomum burmannii and Eleutherine palmifolia extract in high-fat diet (HFD)-induced hyperlipidemia mice.
Materials and Methods: Mice were divided into eight groups (n=4): Control group or healthy mice (normal), HFD-induced hyperlipidemic mice without any treatment (CE0), HFD-induced hyperlipidemic mice treated with 3.6 mg/kg body weight (BW) atorvastatin (atorvastatin), and HFD-induced hyperlipidemic mice treated with a combination of C. burmannii and E. palmifolia in the following ratios: 300:0 (C300), 225:75 (C225), 150:150 (CE150), 75:225 (E225), and 0:300 (E300). Mice were fed a HFD for 4 months to induce hyperlipidemia. Total cholesterol, cholesterol oxidase-peroxidase aminophenazone (CHOD-PAP), triglyceride-glycerine, and fat serum were analyzed with colorimetric method. The measurement of superoxide dismutase was done with the xanthine oxidase method and malondialdehyde measurement was done with the thiobarbituric acid method.
Results: Results showed an increase in antihyperlipidemic characteristics as the concentration of E. palmifolia extract (p<0.05) increased. Duncan's multiple range test also showed an increase in anti-stress oxidation as the concentration of C. burmannii extract (p<0.05) increased.
Conclusion: The E225 group showed the most potential as a safe, antihyperlipidemic agent characterized by improvement in lipid profile and antioxidant balance. Keywords: antihyperlipidemic, Cinnamomum burmannii, Eleutherine palmifolia, lipid profile, malondialdehyde, superoxide dismutase.
Keywords: antihyperlipidemic, Cinnamomum burmannii, Eleutherine palmifolia, lipid profile, malondialdehyde, superoxide dismutase.
How to cite this article: Susilowati R, Setiawan AM (2020) Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice, Veterinary World, 13(7): 1404-1409.
Received: 18-02-2020 Accepted: 29-05-2020 Published online: 22-07-2020
Corresponding author: Retno Susilowati E-mail: retno.susilowati@bio.uin-malang.ac.id
DOI: 10.14202/vetworld.2020.1404-1409
Copyright: Susilowati and Setiawan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.