Vet World Vol.13 July-2020 Article-28
Research Article
Veterinary World, 13(7): 1457-1461
https://doi.org/10.14202/vetworld.2020.1457-1461
Malacca leaf ethanolic extract (Phyllanthus emblica) as a hepatoprotector of the liver of mice (Mus musculus) infected with Plasmodium berghei
2. Clinical Laboratory, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.
3. Pathology Laboratory, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.
4. Parasitology Laboratory, Faculty of Veterinary Medicine Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.
5. Microbiology Laboratory, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.
6. Veterinary Education Study Program, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
Background and Aim: Although existing research confirms the antiparasitic effect of the Malacca plant against Plasmodium, its effect on the liver, one of the target organs of Plasmodium has not been investigated. Therefore, this study was conducted to explore the potential of the ethanolic extract of Malacca (Phyllanthus emblica) leaves in preventing liver damage in mice (Mus musculus) caused by Plasmodium berghei infection.
Materials and Methods: This study was conducted using the livers of 18 mice fixed in 10% neutral-buffered formalin. A completely randomized design with a unidirectional pattern comprising six treatments was used in this study, with each treatment consisting of three replications. Treatment 0 was the negative control group infected with P. berghei, treatment 1 was the positive control group infected with P. berghei followed by chloroquine administration at a dose of 5 mg/kg BW, and treatments 2, 3, 4, and 5 were groups infected with P. berghei and administered Malacca leaf ethanolic extracts at doses of 100, 300, 600, and 1200 mg/kg BW, respectively. The extracts were administered orally using a gastric tube for 4 consecutive days. Mice were sacrificed on the 7th day and livers were collected for histopathological examination.
Results: Histopathological examination of the livers of mice infected with P. berghei demonstrated the presence of hemosiderin, hydropic degeneration, fat degeneration, necrosis, and megalocytosis. However, all these histopathological changes were reduced in the livers of P. berghei-infected mice treated with various doses of Malacca leaf ethanolic extract. The differences between the treatments were found be statistically significant (p<0.05).
Conclusion: Ethanolic extract of Malacca leaves has the potential to protect against liver damage in mice infected with P. berghei. The dose of 600 mg/kg BW was found to be the most effective compared with the doses of 100, 300, and 1200 mg/kg BW. Keywords: hepatoprotector, Malacca leaf extract, megalocytosis, Plasmodium berghei.
Keywords: hepatoprotector, Malacca leaf extract, megalocytosis, Plasmodium berghei.
How to cite this article: Asmilia N, Aliza D, Fahrimal Y, Abrar M, Ashary S (2020) Malacca leaf ethanolic extract (Phyllanthus emblica) as a hepatoprotector of the liver of mice (Mus musculus) infected with Plasmodium berghei. Veterinary World, 13(7): 1457-1461.
Received: 09-01-2020 Accepted: 09-06-2020 Published online: 27-07-2020
Corresponding author: Dwinna Aliza E-mail: dwinna.aliza@unsyiah.ac.id
DOI: 10.14202/vetworld.2020.1457-1461
Copyright: Asmilia, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.