Vet World Vol.13 July-2020 Article-30
Research Article
Veterinary World, 13(7): 1473-1479
https://doi.org/10.14202/vetworld.2020.1473-1479
Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt
2. Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Banha, Egypt.
3. The Reference Laboratory for Quality Control on Poultry Production, Animal Health Research Institute, Dokki, Giza, Egypt.
Background and Aim: Raoultella ornithinolytica is one of the emerging gram-negative bacteria, which associated with foodborne illness. Researches affirmed that distinguish between R. ornithinolytica and Klebsiella oxytoca are difficult, as they are phylogenetic related. The evolution of multidrug resistance of Raoultella strains gained more concern for recognition of the pathogen which supports in controlling the disease and minify its threat. This study sought to find a reliable tool for the identification of Raoultella ornithinolytica, isolated from chicken product samples, and assessed the resistance profile of R. ornithinolytica using antibiogram sensitivity tests.
Materials and Methods: Forty samples of chicken products were collected between January and September 2019 from different markets in Alexandria Governorate, Egypt. The products included nuggets, strips, burgers, luncheon meats, pane, frankfurters, and minced chicken meat. The samples were transferred to the Reference Laboratory. The samples were subjected to isolation, biochemical reaction testing, phenotypic system analytical profile index (API) E20, and a detection of antimicrobial susceptibility test. Phenotypic identification was confirmed through matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).
Results: Thirty-three bacterial isolates (82.50%) out of 40 samples were isolated into pure cultures from the chicken samples. Three isolates (9.09%) were positive for R. ornithinolytica, while 30 isolates (90.91%) exhibited growth characters for different pathogens (Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, R. ornithinolytica, and Klebsiella pneumoniae). The isolates of R. ornithinolytica were resistant to five types of antibiotics and sensitive to two types of antibiotics.
Conclusion: This study reported the first case of R. ornithinolytica found in chicken products in Egypt. Phenotypic system API 20E and MALDI-TOF MS were found to be reliable tools for confirming the diagnosis of R. ornithinolytica. As it provides rapid identification with high sensitivity and specificity for R. ornithinolytica, which often do not require a molecular procedure for confirmation. Keywords: analytical profile index 20E, matrix-assisted laser-desorption ionization time-of-flight mass spectrometry, phenotypic system, Raoultella ornithinolytica.
Keywords: analytical profile index 20E, matrix-assisted laser-desorption ionization time-of-flight mass spectrometry, phenotypic system, Raoultella ornithinolytica.
How to cite this article: El-Shannat SM, El-Tawab AAA, Hassan WMM (2020) Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt, Veterinary World, 13(7): 1473-1479.
Received: 04-03-2020 Accepted: 04-06-2020 Published online: 29-07-2020
Corresponding author: Sara M. El-Shannat E-mail: saramvet@yahoo.com
DOI: 10.14202/vetworld.2020.1473-1479
Copyright: El-Shannat, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.