A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats

Vet World Vol.13 July-2020 Article-5

Research Article

Veterinary World, 13(7): 1280-1287

https://doi.org/10.14202/vetworld.2020.1280-1287

Kalbaza Ahmed Yassine^1,2, Hemida Houari^2,3, Benchohra Mokhtar^2,3, Amara Karim^2,3, Salem Hadjer¹, and Bediaf Imane¹

1. Department of Veterinary Sciences, Institute of Veterinary and Agronomic Sciences, University of BATNA-1, Algeria.
2. Laboratory of Agro-Biotechnology and Nutrition in Semi-Arid Regions, University of Tiaret, Algeria.
3. Department of Animal Health, Institute of Veterinary Sciences, University of Tiaret, Algeria.

Background and Aim: Lawsonia inermis (LI), a naturally grown or cultivated shrub in Northeast of Africa and India, has been traditionally used as a strong remedy for several injuries. However, few studies have reported its use as a cicatrizing agent. The aim of this study was to evaluate the effect of daily application of an ointment prepared with LI leaves' powder on wound healing in Wistar rats.

Materials and Methods: Twenty female Wistar rats were used in this study. Excisional wound model was realized by removing skin from the dorsal part of the neck of each animal. Wounds have been then treated by a daily application of LI ointment prepared by mixing leaves' powder to petroleum jelly in test group and by simple application of petroleum jelly in control group. Evaluation of wound healing activity was then based on calculating the percentage of wound contraction, period of epithelialization, and wound index every 3 days for a period of 24 days, then, a histological study of the healed excised wound was performed.

Results: Treatment with LI has shown excellent wound healing activity, since it has increased percent of wound contraction, and reduced period of epithelialization and wound index as compared to control (p<0.05). These results have been supported by the histological findings that revealed better epithelialization, dermal differentiation, collagen fiber orientation, and angiogenesis in LI treated rats compared to control (p<0.05).

Conclusion: We can conclude that LI leaves' can be used as a potential wound healing agent. Keywords: excision, Lawsonia inermis, petroleum jelly, Wistar rats, wound healing.

Keywords: excision, Lawsonia inermis, petroleum jelly, Wistar rats, wound healing.

How to cite this article: Yassine KA, Houari H, Mokhtar B, Karim A, Hadjer S, Imane B (2020) A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats, Veterinary World, 13(7): 1280-1287.

Received: 31-01-2020 Accepted: 15-05-2020 Published online: 07-07-2020

Corresponding author: Kalbaza Ahmed Yassine E-mail: yassine.kalbaza@univ-batna.dz

DOI: 10.14202/vetworld.2020.1280-1287

Copyright: Yassine, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.