Vet World Vol.13 July-2020 Article-9
Research Article
Veterinary World, 13(7): 1311-1318
https://doi.org/10.14202/vetworld.2020.1311-1318
Deleterious effect of short-term gavage of an ethanol extract of cogon grass (Imperata cylindrica L.) roots on testis and epididymal sperm quality
2. Central Laboratory, Universitas Padjadjaran, Jl. Raya Bandung - Sumedang km. 21, Jatinangor Sumedang, West Java, Indonesia.
3. Department of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Indonesia.
4. Centre for Biotechnology and Biodiversity Research and Development, Universitas Sebelas Maret, Surakarta, Indonesia.
5. Physiology, Developmental Biology and Biomedical Science Research Group, School of Life Science and Technology, Bandung Institute of Technology, Bandung, West Java, Indonesia.
6. Under Graduate Medical Study Program, Faculty of Medicine, Universitas Padjajaran, Jl. Raya Bandung - Sumedang km. 21, Jatinangor Sumedang, West Java, Indonesia.
7. Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Jl. Raya Bandung - Sumedang km. 21, Jatinangor Sumedang, West Java, Indonesia.
8. Department of Obstetrics and Gynecology, Faculty of Medicine, Udayana University, Jl. PB. Sudirman, Denpasar, Bali, Indonesia.
Background and Aim: Cogon grass (Imperata cylindrica L.) (CGG) is a herbal medicine that could be developed into a male antifertility agent. The present study aims to determine the effect of an ethanol extract of CGG roots on mice testicular activity, reproductive hormone levels, and epididymal sperm quality.
Materials and Methods: This study was designed as completely randomized with three different doses, such as an ethanol extract of CGG roots at 0 (control), 90, and 115 mg/kg body weight. In total, 21 male DDY mice strain were treated with the CGG extract (by gavage) for 14 days, followed by an evaluation of reproductive organs, epididymal sperm quality, testis histology, histomorphometry, and reproductive hormone assays. All quantitative data were analyzed by analysis of variance, followed by Tukey's post hoc test at α=0.05.
Results: The results showed that the administration of the CGG root ethanol extract disrupted the testis interstitial area and seminiferous tubules, resulting in decreased epididymal sperm quality as well as serum testosterone levels in a dose-dependent pattern.
Conclusion: Oral administration of a CGG root ethanol extract induced testicular damage, decreased epididymal sperm quality, and impaired testosterone secretion. Keywords: cogon grass root ethanol extract, epididymal sperm quality, male antifertility, reproductive hormones.
Keywords: cogon grass root ethanol extract, epididymal sperm quality, male antifertility, reproductive hormones.
How to cite this article: Widyastuti R, Prastowo S, Sumarsono SH, Lubis A, Hartady T, Syamsunarno MRAA, Sudiman J (2020) Deleterious effect of short-term gavage of an ethanol extract of cogon grass (Imperata cylindrica L.) roots on testis and epididymal sperm quality, Veterinary World, 13(7): 1311-1318.
Received: 06-02-2020 Accepted: 19-05-2020 Published online: 13-07-2020
Corresponding author: Sigit Prastowo E-mail: prastowo@staff.uns.ac.id
DOI: 10.14202/vetworld.2020.1311-1318
Copyright: Widyastuti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.