Vet World Vol.13 March-2020 Article-18
Research Article
Veterinary World, 13(3): 515-520
https://doi.org/10.14202/vetworld.2020.515-520
Acute and subacute toxicity tests of goat bile in BALB/c mice
2. HVA Hospital, Pare, Kediri, Indonesia.
3. Master Program of Immunology, Postgraduate School, Universitas Airlangga, Surabaya, Indonesia.
4. Department of Pharmacology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
5. Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
6. Dr. Soetomo Hospital, Surabaya, Indonesia.
7. Department of Anatomic Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Background and Aim: The aim of this study was to investigate the toxicity of goat bile in BALB/c mice since some Indonesian people consume raw goat gallbladder to treat malaria and increase stamina.
Materials and Methods: Acute toxicity test was done in six groups of BALB/c mice using 100%, 50%, 25%, 12.5%, and 6.75% of goat bile and negative control. The death of mice was observed within 14 days. In the subacute toxicity test, the body weight and hematology parameters on day 0 and day 4 post-treatment were evaluated. The mice were closely observed for 28 days before plasma collection for the blood biochemistry evaluation.
Results: Mild diarrhea was observed in acute and subacute toxicity tests. No death of mice was observed in acute test. Goat bile did not inhibit the increase of the body weight of mice. A slight reduction in hemoglobin and hematocrit levels in mice treated with 25% and 50% goat bile, however, remained normal in mice treated with 100% goat bile. The red and white blood cell count were not affected. Liver and kidney functions were not affected by goat bile treatment as revealed by the plasma level of aspartate aminotransferase and alanine aminotransferase, blood urea nitrogen, and creatinine, which remained in the normal range.
Conclusion: Goat bile treatment in BALB/c mice caused mild toxicity in mice. Hydrophobic bile acids may cause the toxicity of goat bile in mice; therefore, it is recommended that goat bile consumption not to be taken oftenly to avoid its harmful effect. Keywords: BALB/c mice, goat bile, Indonesia, toxicity.
Keywords: BALB/c mice, goat bile, Indonesia, toxicity.
How to cite this article: Arwati H, Hapsari WT, Wardhani KA, Aini KN, Bahalwan RR, Wardhani P, Sandhika W (2020) Acute and subacute toxicity tests of goat bile in BALB/c mice, Veterinary World, 13(3): 515-520.
Received: 22-10-2019 Accepted: 06-02-2020 Published online: 20-03-2020
Corresponding author: Heny Arwati E-mail: heny-a@fk.unair.ac.id
DOI: 10.14202/vetworld.2020.515-520
Copyright: Arwati, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.