Vet World Vol.13 May-2020 Article-7
Research Article
Veterinary World, 13(5): 879-883
https://doi.org/10.14202/vetworld.2020.879-883
Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction
2. Department of Veterinary Clinical, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Background and Aim: The follicle-stimulating hormone (FSH) gene is an essential regulator of fertility in livestock. This study aims to provide information on the genetic makeup of Madrasin cattle experiencing hypofunction by the FSH profile and FSH receptors (FSHR) polymorphism.
Materials and Methods: Blood samples were collected from the Bangkalan regency in Indonesia. DNA was isolated and purified following the extraction protocol of polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism.
Results: Our results showed that the FSH gene had a band length of 310 bp and produce two alleles (A and B) with restriction enzymes at 250 bp, 230 bp, and 145 bp. Furthermore, the FSHR gene had a band length of 303 bp and produced two homozygous genotypes: GG at bp 239 and CC at bp 188.
Conclusion: Based on these differences, there was no change in allele frequency and genotype between Madura and Madrasin cattle due to crossbreeding with Limousin cattle. Thus, further detailed investigations of Madrasin cattle are required to elucidate the profile of the LH and LHR genes. Keywords: follicle-stimulating hormone, follicle-stimulating hormone receptors, hypofunction, Madrasin.
Keywords: follicle-stimulating hormone, follicle-stimulating hormone receptors, hypofunction, Madrasin.
How to cite this article: Utomo B, Putranto ED, Fadholly A (2020) Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction, Veterinary World, 13(5): 879-883.
Received: 18-10-2019 Accepted: 07-04-2020 Published online: 11-05-2020
Corresponding author: Budi Utomo E-mail: budi_reprovet@yahoo.com
DOI: 10.14202/vetworld.2020.879-883
Copyright: Utomo, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.