Vet World Vol.13 November-2020 Article-24
Research Article
Veterinary World, 13(11): 2459-2468
https://doi.org/10.14202/vetworld.2020.2459-2468
Characterization of Burkholderia pseudomallei from spontaneous melioidosis in a Bornean orangutan
2. Primate Research Center, IPB University, Bogor 16128, Indonesia.
3. Borneo Orangutan Survival Foundation, Bogor 16128, Indonesia.
4. Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia.
5. Department of Clinics, Reproduction, and Pathology, Faculty of Veterinary Medicine, IPB University, Bogor 16680, Indonesia.
6. Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor 16680, Indonesia.
Background and Aim: Melioidosis is a potentially fatal disease affecting humans and a wide range of animal species; it is often underdiagnosed and underreported in veterinary medicine in Indonesia. This study aimed to characterize morphological and molecular features of Burkholderia pseudomallei, the causative agent of melioidosis which caused the death of a Bornean orangutan.
Materials and Methods: Pulmonary abscess samples were cultured on several types of media, including Ashdown agar, Ashdown broth, and MacConkey agar. Type three secretion system orf 2 real-time polymerase chain reaction (PCR) and latex agglutination tests were performed to identify the bacteria. Morphological characteristics were compared to all previously published morphotypes. Subsequently, the bacteria were characterized by multilocus sequence typing (MLST) and Yersinia-like flagellum/Burkholderia thailandensis-like flagellum and chemotaxis PCR. The results of the genotyping were afterward compared to all genotypes from Southeast Asia.
Results: Multiple morphotypes of B. pseudomallei were perceived during the growth on Ashdown agar. Furthermore, it was identified by MLST that the Type I and Type II morphotypes observed in this study were clones of a single ST, ST54, which is predominantly found in humans and the environment in Malaysia and Thailand, although a very limited number of reports was published in association with animals. Moreover, the E-BURST analysis showed that the ST is grouped together with isolates from Southeast Asian countries, including Malaysia, Thailand, Singapore, and Cambodia. ST54 was predicted to be the founding genotype of several STs from those regions.
Conclusion: B. pseudomallei ST54 that caused the death of a Bornean orangutan has a distant genetic relationship with other STs which were previously reported in Indonesia, implying a vast genetic diversity in Indonesia that has not been discovered yet. Keywords: Burkholderia pseudomallei, melioidosis, molecular characterization, nonhuman primate, orangutan.
Keywords: Burkholderia pseudomallei, melioidosis, molecular characterization, nonhuman primate, orangutan.
How to cite this article: Testamenti VA, Surya M, Saepuloh U, Iskandriati D, Tandang MV, Kristina L, Wahyudi AT, Sajuthi D, Santi VD, Patispathika FH, Wahyu M, Nurcahyo A, Pamungkas J (2020) Characterization of Burkholderia pseudomallei from spontaneous melioidosis in a Bornean orangutan, Veterinary World, 13(11): 2459-2468.
Received: 29-03-2020 Accepted: 13-10-2020 Published online: 18-11-2020
Corresponding author: Joko Pamungkas E-mail: jpi-pssp@indo.net.id
DOI: 10.14202/vetworld.2020.2459-2468
Copyright: Testamenti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.