Efficacy of a prepared tissue culture-adapted vaccine against Chlamydia psittaci experimentally in mice

Vet World Vol.13 November-2020 Article-35

Research Article

Veterinary World, 13(11): 2546-2554

https://doi.org/10.14202/vetworld.2020.2546-2554

J. El-Jakee¹, Mahmoud D. El-Hariri¹, Mona A. El-Shabrawy², Afaf A. Khedr³, Riham H. Hedia², Eman A. Khairy², E. S. Gaber², and Eman Ragab¹

1. Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt.
2. Department of Microbiology and Immunology, National Research Center, El Dokki, Cairo, Egypt.
3. Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Abbasia, Cairo, Egypt.

Background and Aim: Chlamydia psittaci is an intracellular pathogen with a broad range of hosts and endemic in nearly all bird species as well as many mammalian species. Outbreaks contribute to economic losses, especially due to infection of pet birds, poultry, and livestock. Worse, the organism has a zoonotic effect, and transmission to humans results in severe illness. Therefore, proper control measures need to be applied. We conducted a trial for the preparation and evaluation of inactivated vaccine against C. psittaci.

Materials and Methods: Three C. psittaci strains (accession nos.: KP942827, KP942828, and KP942829) were grown in embryonated chicken eggs and then propagated for purification in Vero cells. The immunization experiment was experimentally performed in mice, which then were challenged with a virulent C. psittaci strain.

Results: The immunization trial revealed nearly 100% protection after the challenge. The histopathological and immunofluorescence examinations of internal organs revealed that the prepared killed vaccines can effectively reduce chlamydial infection and shedding in animals with the proper level of protection.

Conclusion: Our vaccine can be used to control economic and financial losses resulting from avian chlamydiosis, especially those in poultry industries. The zoonotic transmission risk highlights the need for proper control measures. Keywords: Chlamydia psittaci, human, immunofluorescence, inactivated vaccine, poultry, Vero cell.

Keywords: Chlamydia psittaci, human, immunofluorescence, inactivated vaccine, poultry, Vero cell.

How to cite this article: El-Jakee J, El-Hariri MD, El-Shabrawy MA, Khedr AA, Hedia RH, Khairy EA, Gaber ES, Ragab E (2020) Efficacy of a prepared tissue culture-adapted vaccine against Chlamydia psittaci experimentally in mice, Veterinary World, 13(11): 2546-2554.

Received: 19-08-2020 Accepted: 20-10-2020 Published online: 28-11-2020

Corresponding author: Eman Ragab E-mail: eman_ragab2008@cu.edu.eg

DOI: 10.14202/vetworld.2020.2546-2554

Copyright: El-Jakee, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.