Vet World Vol.13 October-2020 Article-11
Research Article
Veterinary World, 13(10): 2118-2125
https://doi.org/10.14202/vetworld.2020.2118-2125
Whole-genome-based phylogeny of African swine fever virus
2. Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, Yerevan, Armenia.
3. Experimental Laboratory, Yerevan State Medical University, Yerevan, Armenia.
4. Department of Medical Biology, Yerevan State Medical University, Yerevan, Armenia.
Background and Aim: A genome-scale phylogenetic analysis was used to infer the evolutionary dynamics of Asfarviridae – African swine fever virus (ASFV) – and better define its genetic diversity.
Materials and Methods: All complete ASFV genomes from NCBI's resource as of March 2020 were used. The phylogenetic analysis used maximum likelihood and neighbor-joining methods. The evolutionary models detection was done with the help of the package of programs MEGA-X. Algorithms were used to build phylogenetic trees for type B DNA polymerases of ASFV (n=34) and HcDNAV (n=2), as an external group.
Results: An expedient categorization of the Asfarviridae family uses five clades. Genotype 1 (except for LIV 5/40 virus isolate) as well genotype 7 are assigned to the alpha clade; genotype 2 to the beta clade; genotypes 8, 9, and 10 to the gamma clade; genotype 5 to the delta clade; and genotypes 3, 4, and 20, as well as genotype 22 and the LIV 5/40 isolate to the epsilon clade. Branch lengths on the phylogenetic tree are proportional to genetic distance along the branch. Branches at the phylogenetic tree of Asfarviridae are much shorter than branches for Baculoviridae. Shorter branches in ASFVs population suggest that Asfarviridae evolved relatively recently and remain more closely related.
Conclusion: We suggest applying more robust standards using whole genomes to ensure the correct classification of ASFV and maintain phylogeny as a useful tool. Keywords: African swine fever virus, baculovirus, phylogenetic tree.
Keywords: African swine fever virus, baculovirus, phylogenetic tree.
How to cite this article: Aslanyan L, Avagyan H, Karalyan Z (2020) Whole-genome-based phylogeny of African swine fever virus, Veterinary World, 13(10): 2118-2125.
Received: 14-05-2020 Accepted: 21-08-2020 Published online: 10-10-2020
Corresponding author: Hranush Avagyan E-mail: a.avagian@yahoo.com
DOI: 10.14202/vetworld.2020.2118-2125
Copyright: Aslanyan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.