Vet World Vol.13 October-2020 Article-8
Research Article
Veterinary World, 13(10): 2097-2103
https://doi.org/10.14202/vetworld.2020.2097-2103
The distribution pattern and growth factor level in platelet-rich fibrin incorporated skin-derived mesenchymal stem cells: An in vitro study
2. Stem Cell Research and Development Center, Airlangga University, Surabaya, Indonesia.
3. Doctoral Student of Medical Science, Faculty of Medicine, Airlangga University, Surabaya, Indonesia.
4. Department of Health, Vocational Faculty, Airlangga University, Surabaya, Indonesia.
5. Department of Pharmacology, Veterinary Medicine Faculty, Airlangga University, Surabaya, Indonesia.
6. Department of Microbiology, Virology Laboratory, Veterinary Medicine Faculty, Airlangga University, Surabaya, Indonesia.
Background and Aim: A skin wound in an animal must be cared for to prevent further health issues. Platelet-rich fibrin (PRF) and skin-derived mesenchymal stem cells (SMSCs) have been reported to have potential in increasing the rate of wound healing. This study aimed to analyze the distribution patterns and levels of platelet-derived growth factor (PDGF), insulin-like growth factor (IGF), vascular endothelial growth factor (VEGF), and transforming growth factor-β (TGF-β) in PRF incorporated with SMSCs.
Materials and Methods: This study employed a true experiment (in vitro) design with post-test only performed in the control group alone. PRF and SMSCs were extracted from the blood and skin of 16 rabbits. SMSCs were characterized using immunocytochemistry to examine clusters of differentiation for 45, 73, 90, and 105. PRF was incorporated into the SMSCs and then divided into four groups (N=32/n=8): Group A (PRF only), Group B (PRF+SMSCs, incubated for 1 day), Group C (PRF+SMSCs, incubated for 3 days), and Group D (PRF+SMSCs, incubated for 5 days). Scanning electron microscopy was used to examine the distribution pattern of SMSCs between groups. The supernatant serum (Group A) and supernatant medium culture (Group D) were collected for the measurement of PDGF, IGF, VEGF, and TGF-β using an enzyme-linked immunosorbent assay sandwich kit. An unpaired t-test was conducted to analyze the differences between Groups A and D (p<0.01).
Results: Group D had the most morphologically visible SMSCs attached to the PRF, with elongated and pseudopodia cells. There was a significant difference between the levels of growth factor in Groups A and D (p=0.0001; p<0.01).
Conclusion: SMSCs were able to adhere to and distribute evenly on the surface of PRF after 5 days of incubation. The PRF incorporated SMSCs contained high levels of PDGF, IGF, VEGF, and TGF- β, which may prove to have potential in enhancing wound healing. Keywords: growth factor, platelet-rich fibrin, rabbit, skin mesenchymal stem cells.
Keywords: growth factor, platelet-rich fibrin, rabbit, skin mesenchymal stem cells.
How to cite this article: Ihsan IS, Karsari D, Ertanti N, Dinaryanti A, Nugraha AP, Purwati P, Sudjarwo SA, Rantam FA (2020) The distribution pattern and growth factor level in platelet-rich fibrin incorporated skin-derived mesenchymal stem cells: An in vitro study, Veterinary World, 13(10): 2097-2103.
Received: 15-05-2020 Accepted: 31-08-2020 Published online: 07-10-2020
Corresponding author: Fedik Abdul Rantam E-mail: fedik-a-r@fkh.unair.ac.id
DOI: 10.14202/vetworld.2020.2097-2103
Copyright: Ihsan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.