Vet World Vol.13 September-2020 Article-2
Research Article
Veterinary World, 13(9): 1743-1749
https://doi.org/10.14202/vetworld.2020.1743-1749
Listeriosis in a peri-urban area: Cultural and molecular characterization of Listeria monocytogenes isolated from encephalitic goats
2. Department of Microbiology, Lakhimpur College of Veterinary Science, Assam Agricultural University, Joyhing, North Lakhimpur, Assam, India.
3. Department of Pathology, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
4. Department of Parasitology, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
5. Teaching Veterinary Clinical Complex, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
Background and Aim: Listeriosis in food animals bears a significant threat to human health. Detailed investigations into the cause facilitate proper management of the disease. This study reports the cultural, pathological, and molecular characterization of Listeria monocytogenes isolated from encephalitic goats from peri-urban Guwahati, Assam.
Materials and Methods: Out of nine suspected samples, five positive isolates of L. monocytogenes were subjected to bacteriological, biochemical, and molecular tests. The genus and species-specific L. monocytogenes 16S rRNA and prs genes were amplified by polymerase chain reaction (PCR) to yield 1200 and 370 bp sized products, respectively. The encephalitic form of the disease was characterized by circling movement, high fever, and terminal recumbence.
Results: All the five isolates were confirmed to be L. monocytogenes based on PCR amplification of genus and species-specific 16S rRNA and prs gene products. The isolates were sensitive to ciprofloxacin, oxytetracycline (OTC), and norfloxacin, but resistant to doxycycline and erythromycin. A high dose of OTC was used in a goat at the early stage of clinical symptom and the animal recovered clinically.
Conclusion: Listeriosis in goats could pose a significant public health threat as the meat (occasionally milk) or meat products from goats are widely consumed by the people of Assam. Understanding the molecular epidemiological aspects of L. monocytogenes infections of food animal species should, therefore, be the priority in this part of the country. Keywords: antibiogram, Assam, goat, Listeria monocytogenes, polymerase chain reaction.
Keywords: antibiogram, Assam, goat, Listeria monocytogenes, polymerase chain reaction.
How to cite this article: Barman NN, Nath AJ, Doley S, Begum SA, Kakati P, Das SK, Rahman T, Bhuyan D, Baishya BC, Goswami S (2020) Listeriosis in a peri-urban area: Cultural and molecular characterization of Listeria monocytogenes isolated from encephalitic goats, Veterinary World, 13(9): 1743-1749.
Received: 28-04-2020 Accepted: 14-07-2020 Published online: 02-09-2020
Corresponding author: Nagendra Nath Barman E-mail: nnbarman@gmail.com
DOI: 10.14202/vetworld.2020.1743-1749
Copyright: Barman, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.