Vet World Vol.13 September-2020 Article-21
Research Article
Veterinary World, 13(9): 1898-1901
https://doi.org/10.14202/vetworld.2020.1898-1901
First report of PCR-based detection of Helicobacter species DNA in Camelus dromedarius in Egypt
2. Department of Microbiology (Bacteriology), Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
3. Department of Biotechnology, Animal Health Research Institute, 7 Nady El Seid St., Dokki, Giza, Egypt.
Background and Aim: Helicobacter species infections have epidemiological and zoonotic impacts, and different species of Helicobacter have been implicated in infecting humans and animals. The aim of this study was to investigate Helicobacter species infections in Camelus dromedarius.
Materials and Methods: Fecal samples were collected from 32 camels from 9 camel farms located at Ismailia Governorate, Egypt. The collected samples were investigated by bacteriological isolation and conventional polymerase chain reaction (PCR) assays targeting the 16S ribosomal RNA gene.
Results: Although Helicobacter species could not be isolated from all the examined samples, Helicobacter DNA was detected in 2 (22.22%) of the 9 camel farms. Of the 32 camel fecal samples examined, 4 (12.5%) were positive for Helicobacter species as analyzed by the PCR assay.
Conclusion: To the best of our knowledge, this is the first report of PCR-based detection of Helicobacter species infections in C. dromedarius. Further epidemiological studies are required to clarify Helicobacter species infections in camels. Keywords: Camelus dromedarius, Helicobacter, Non-pylori, polymerase chain reaction.
Keywords: Camelus dromedarius, Helicobacter, Non-pylori, polymerase chain reaction.
How to cite this article: Youssef A, Afifi A, Hamed A, Enany M (2020) First report of PCR-based detection of Helicobacter species DNA in Camelus dromedarius in Egypt, Veterinary World, 13(9): 1898-1901.
Received: 14-03-2020 Accepted: 10-07-2020 Published online: 17-09-2020
Corresponding author: Ahmed Youssef E-mail: ayoussef22.com@gmail.com
DOI: 10.14202/vetworld.2020.1898-1901
Copyright: Youssef, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.