Prevalence and antibiotic susceptibility of Mannheimia haemolytica and Pasteurella multocida isolated from ovine respiratory infection: A study from Karnataka, Southern India

Vet World Vol.13 September-2020 Article-28

Research Article

Veterinary World, 13(9): 1947-1954

https://doi.org/10.14202/vetworld.2020.1947-1954

Swati Sahay^1,2, Krithiga Natesan¹, Awadhesh Prajapati¹, Triveni Kalleshmurthy¹, Bibek Ranjan Shome¹, Habibur Rahman³, and Rajeswari Shome¹

1. Indian Council of Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India.
2. Department of Microbiology, Centre for Research in Pure and Applied Sciences, Jain University, Bengaluru, Karnataka, India.
3. International Livestock Research Institute, CG Centre, NASC Complex, DPS Marg, Pusa, New Delhi, India.

Background and Aim: Respiratory infection due to Mannheimia haemolytica and Pasteurella multocida are responsible for huge economic losses in livestock sector globally and it is poorly understood in ovine population. The study aimed to investigate and characterize M. haemolytica and P. multocida from infected and healthy sheep to rule out the involvement of these bacteria in the disease.

Materials and Methods: A total of 374 healthy and infected sheep samples were processed for isolation, direct detection by multiplex PCR (mPCR), and antibiotic susceptibility testing by phenotypic and genotypic methods.

Results: Overall, 55 Pasteurella isolates (27 [7.2%] M. haemolytica and 28 [7.4%] P. multocida) were recovered and identified by bacteriological tests and species-specific PCR assays. Significant correlation between the detection of M. haemolytica (66.6%) with disease condition and P. multocida (19.1%) exclusively from infected sheep was recorded by mPCR. In vitro antibiotic susceptibility testing of 55 isolates revealed higher multidrug resistance in M. haemolytica (25.9%) than P. multocida (7.1%) isolates. Descending resistance towards penicillin (63.6%), oxytetracycline (23.6%), streptomycin (14.5%), and gentamicin (12.7%) and absolute sensitivity towards chloramphenicol were observed in both the pathogens. The antibiotic resistance genes such as strA (32.7%) and sul2 (32.7%) associated with streptomycin and sulfonamide resistance, respectively, were detected in the isolates.

Conclusion: The study revealed the significant involvement of M. haemolytica together with P. multocida in ovine respiratory infection and is probably responsible for frequent disease outbreaks even after vaccination against hemorrhagic septicemia in sheep population of Karnataka, southern province of India. Keywords: antimicrobial susceptibility, isolation, Mannheima haemolytica, multiplex PCR, Pasteurella multocida.

Keywords: antimicrobial susceptibility, isolation, Mannheima haemolytica, multiplex PCR, Pasteurella multocida.

How to cite this article: Sahay S, Natesan K, Prajapati A, Kalleshmurthy T, Shome BR, Rahman H, Shome R (2020) Prevalence and antibiotic susceptibility of Mannheimia haemolytica and Pasteurella multocida isolated from ovine respiratory infection: A study from Karnataka, Southern India, Veterinary World, 13(9): 1947-1954.

Received: 25-04-2020 Accepted: 29-07-2020 Published online: 23-09-2020

Corresponding author: Rajeswari Shome E-mail: rajeswarishome@gmail.com

DOI: 10.14202/vetworld.2020.1947-1954

Copyright: Sahay, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.