Vet World Vol.13 September-2020 Article-39
Research Article
Veterinary World, 13(9): 2028-2032
https://doi.org/10.14202/vetworld.2020.2028-2032
Ocimum sanctum Linn. ethanolic extract inhibits angiogenesis in human lung adenocarcinoma (a549) cells
2. Integrated Laboratory for Research and Testing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
3. Department of Clinical Pathology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
4. Department of Oral Medicine, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta Indonesia.
Background and Aim: Ocimum sanctum (OS) is a herbal plant, which is easy to find and is widely used as an alternative medication. The previous studies have shown that several species of OS extract have therapeutic properties, and in some cases, antitumor properties. Furthermore, several data have shown the antiproliferative effects of OS extract in cases of breast cancer, human fibrosarcoma, and oral cancer. Lung adenocarcinoma is a major cause of male cancer worldwide; however, the effect of OS (of Indonesian origin) on the metastasis of human alveolar pulmonary adenocarcinoma A549 cells remains unclear. This study aimed to analyze the antiangiogenic effects of OS ethanolic extract in A549 lung adenocarcinoma cells.
Materials and Methods: An angiogenesis assay was performed by seeding A549 cells on extracellular matrix solution and observing tube formation using an inverted microscope. Enzyme-linked immunosorbent assay for αvβ3, matrix metalloproteinase (MMP)-2, and MMP-9 was performed by analyzing the cell lysate after a given treatment.
Results: OS ethanolic extract significantly inhibited tube formation of A549 cells and suppressed the expression of integrin αvβ3, MMP-2, and MMP-9.
Conclusion: Our findings indicate that OS ethanolic extract disrupts angiogenesis of A549 cells, which may result from the disruption of cell migration and proliferation as a consequence of downregulation of αvβ3, MMP-2, and MMP-9. Taken together, OS ethanolic extract may represent a good therapeutic candidate for the treatment of metastasis in lung adenocarcinoma. Further studies are warranted to further establish the efficacy of OS in the treatment of lung adenocarcinoma. Keywords: A549 cells, angiogenesis, integrin αvβ3, matrix metalloproteinase, Ocimum sanctum ethanolic extract.
Keywords: A549 cells, angiogenesis, integrin αvβ3, matrix metalloproteinase, Ocimum sanctum ethanolic extract.
How to cite this article: Wihadmadyatami H, Hening P, Kustiati U, Kusindarta DL, Triyono T, Supriatno S (2020) Ocimum sanctum Linn. ethanolic extract inhibits angiogenesis in human lung adenocarcinoma (a549) cells, Veterinary World, 13(9):2028-2032.
Received: 01-04-2020 Accepted: 31-07-2020 Published online: 30-09-2020
Corresponding author: Hevi Wihadmadyatami E-mail: heviwihadmadyatami@ugm.ac.id
DOI: 10.14202/vetworld.2020.2028-2032
Copyright: Wihadmadyatami, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.