Vet World Vol.13 September-2020 Article-9
Research Article
Veterinary World, 13(9): 1807-1814
https://doi.org/10.14202/vetworld.2020.1807-1814
Phylogenetic group determination and plasmid virulence gene profiles of colistin-resistant Escherichia coli originated from the broiler meat supply chain in Bogor, Indonesia
2. National Veterinary Drug Assay Laboratory, Directorate General of Livestock and Animal Health, Ministry of Agriculture of the Republic of Indonesia, Indonesia.
Background and Aim: Pathogenic Escherichia coli contamination along the broiler meat supply chain is a serious public health concern. This bacterial infection with multidrug-resistant can lead to treatment failure. Several studies have revealed that avian pathogenic E. coli (APEC) and human extraintestinal pathogenic E. coli (ExPEC) showed a close genetic relationship and may share virulence genes. This study aimed to determine the phylogenetic group and virulence gene profiles in colistin-resistant E. coli obtained from the broiler meat supply chain in Bogor, West Java, Indonesia.
Materials and Methods: Fifty-eight archive isolates originated from the cloacal swab, litter, drinking water, inside plucker swab, fresh meat at small scale poultry slaughterhouses, and traditional markets were used in this study. All the isolates were characterized by a polymerase chain reaction to determine the phylogenetic group (A, B1, B2, or D) and virulence gene profiles with APEC marker genes (iutA, hlyF, iss, iroN, and ompT).
Results: Phylogenetic grouping revealed that the isolates belong to A group (34.48%), D group (34.48%), B1 group (17.24%), and B2 group (13.79%). The virulence gene prevalence was as follows: iutA (36%), hlyF (21%), ompT (21%), iroN (10%), and iss (9%). The B2 group presented with more virulence genes combinations. iroN, hlyF, and ompT genes were positively associated with the B2 group (p≤0.05).
Conclusion: Our results highlight the role of colistin-resistant E. coli originated from the broiler meat supply chain as a potential reservoir for human ExPEC virulence genes. Keywords: broiler supply chain, Escherichia coli, phylogenetic group, virulence gene.
Keywords: broiler supply chain, Escherichia coli, phylogenetic group, virulence gene.
How to cite this article: Rahayuningtyas I, Indrawati A, Wibawan IWT, Palupi MF, Istiyaningsih I (2020) Phylogenetic group determination and plasmid virulence gene profiles of colistin-resistant Escherichia coli originated from the broiler meat supply chain in Bogor, Indonesia, Veterinary World, 13(9): 1807-1814.
Received: 13-04-2020 Accepted: 21-07-2020 Published online: 05-09-2020
Corresponding author: Agustin Indrawati E-mail: titin.seta@gmail.com
DOI: 10.14202/vetworld.2020.1807-1814
Copyright: Rahayuningtyas, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.