Vet World   Vol.14   August-2021  Article-17

Research Article

Veterinary World, 14(8): 2109-2117

https://doi.org/10.14202/vetworld.2021.2109-2117

Wound healing potential of human umbilical cord mesenchymal stem cell conditioned medium: An in vitro and in vivo study in diabetes-induced rats

Siufui Hendrawan1,2, Yuyus Kusnadi3, Christine Ayu Lagonda3, Dilafitria Fauza3, Jennifer Lheman2, Erwin Budi2, Brian Saputra Manurung2, Hans Ulrich Baer4,5, and Sukmawati Tansil Tan6
1. Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tarumanagara University, 11440, Jakarta, Indonesia.
2. Tarumanagara Human Cell Technology Laboratory, Tarumanagara University, 11440, Jakarta, Indonesia.
3. Stem Cell Division, Stem Cell and Cancer Institute, PT. Kalbe Farma, Tbk., 10510, Jakarta, Indonesia.
4. Baermed, Centre of Abdominal Surgery, Hirslanden Clinic, 2501, Zürich, Switzerland.
5. Department of Visceral and Transplantation Surgery, University of Bern, 3012, Bern, Switzerland.
6. Department of Dermatovenereology, Faculty of Medicine, Tarumanagara University, 11440, Jakarta, Indonesia.

Background and Aim: Human umbilical cord mesenchymal stem cells (hUC-MSCs) and its conditioned medium (CM) promote wound healing. This study investigated the wound healing potential of hUC-MSC CM in vitro and in vivo using diabetic animal models.

Materials and Methods: The CM from hUC-MSC CM prepared under hypoxic conditions (hypoxic hUC-MSC) was evaluated for stimulating rat fibroblast growth, collagen production (in vitro), and wound healing in animal models (in vivo). An excision wound on the dorsal side of the diabetes-induced rats was established, and the rats were randomly divided into non-treatment, antibiotic, and hypoxic hUC-MSC CM groups. The cell number of fibroblasts and collagen secretion was evaluated and compared among the groups in an in vitro study. By contrast, wound size reduction, width of re-epithelialization, and the collagen formation area were assessed and compared among the groups in an in vivo study.

Results: CM under hypoxic conditions contained a higher concentration of wound healing-related growth factors. Hypoxic hUC-MSC CM could facilitate fibroblast cell growth and collagen synthesis, although not significant compared with the control group. Re-epithelialization and collagen production were higher in the hUC-MSC CM group than in the antibiotic and non-treatment groups.

Conclusion: Hypoxic hUC-MSC CM possessed more positive effects on the wound healing process based on re-epithelialization and collagen formation than antibiotic treatment did. Keywords: conditioned medium, diabetic induced rat, human umbilical cord mesenchymal stem cells, wound healing.

Keywords: conditioned medium, diabetic induced rat, human umbilical cord mesenchymal stem cells, wound healing.

How to cite this article: Hendrawan S, Kusnadi Y, Lagonda CA, Fauza D, Lheman J, Budi E, Manurung BS, Baer HU, Tan ST (2021) Wound healing potential of human umbilical cord mesenchymal stem cell conditioned medium: An in vitro and in vivo study in diabetes-induced rats, Veterinary World, 14(8): 2109-2117.

Received: 01-03-2021  Accepted: 08-07-2021     Published online: 17-08-2021

Corresponding author: Siufui Hendrawan   E-mail: siufui@gmail.com

DOI: 10.14202/vetworld.2021.2109-2117

Copyright: Hendrawan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.