Vet World Vol.14 August-2021 Article-19
Research Article
Veterinary World, 14(8): 2124-2130
https://doi.org/10.14202/vetworld.2021.2124-2130
Correlation between age, testosterone and adiponectin concentrations, and sperm abnormalities in Simmental bulls
2. Animals Science Program, Faculty of Agriculture, Djuanda University, Jl. Tol Jagorawi No.1, Ciawi, Bogor 16720, Indonesia.
3. Research Center for Biotechnology, Indonesia Institute of Sciences (LIPI), Jl. Raya Jakarta- Bogor, Cibinong, Bogor 16911, Indonesia.
4. Department of Clinic, Reproduction, and Pathology, Faculty of Veterinary Medicine, IPB University, Jl. Agatis, Dramaga Campus, Bogor 16680, Indonesia.
Background and Aim: Capacity for sperm production is affected by age, which is related to the morphology of sperm abnormalities and can affect fertility. The aim of this study was to evaluate the relationship between age and concentrations of testosterone and adiponectin with sperm abnormalities in Simmental bulls.
Materials and Methods: The study used 11 bulls, separated into three groups. The first group consisted of five bulls aged 4-5 years, and the second and third groups each consisted of three bulls, aged 6-7 and 8-10 years, respectively. The average sperm motility of the animals ranged from 57.66±2.60% to 70.17±0.22%. Blood samples were obtained from the coccygeal region of the animals. Testosterone and adiponectin analysis was performed using the enzyme-linked immunosorbent assay method. Sperm morphology was evaluated using carbol fuchsin-eosin staining according to the Williams method. Finally, correlations between testosterone and adiponectin concentrations, age, and sperm abnormalities were analyzed using Pearson's correlation analysis.
Results: The findings revealed a significant correlation (p<0.01) between the concentrations of testosterone and adiponectin (–0.538), age (–0.588), and abnormal sperm morphology (–0.912). Moreover, they revealed that the concentration of testosterone in the bulls aged 8-10 years was lower, at 21.89±4.56 ng/mL, compared to that in the bulls aged 4-5 years, at 36.15±1.29 ng/mL, and 6-7 years, at 35.16±5.39 ng/mL. The findings also revealed a positive correlation between adiponectin concentration and age (0.529) and sperm abnormalities (0.506). The increase in testosterone concentration was inversely related to the adiponectin concentration (–0.538). Moreover, the mean amount of abnormal sperm increased with increasing age: 3.82±0.33% in the group aged 4-5 years, and 4.40±0.72% and 10.20±1.97% in the groups aged 6-7 years and 8-10 years, respectively.
Conclusion: The study data indicate that there is a decrease in testosterone concentration, a high adiponectin concentration, and an increase in abnormal sperm with increasing age in bulls. Keywords: adiponectin, age, Simmental bull, sperm abnormalities, testosterone.
Keywords: adiponectin, age, Simmental bull, sperm abnormalities, testosterone.
How to cite this article: Baharun A, Said S, Arifiantini RI, Karja NWK (2021) Correlation between age, testosterone and adiponectin concentrations, and sperm abnormalities in Simmental bulls, Veterinary World, 14(8): 2124-2130.
Received: 30-03-2021 Accepted: 09-07-2021 Published online: 18-08-2021
Corresponding author: Raden Iis Arifiantini E-mail: arifiantini@apps.ipb.ac.id
DOI: 10.14202/vetworld.2021.2124-2130
Copyright: Baharun, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.