Vet World Vol.14 August-2021 Article-22
Research Article
Veterinary World, 14(8): 2150-2154
https://doi.org/10.14202/vetworld.2021.2150-2154
Characterization of serum protein electrophoresis patterns and C-reactive protein in canine tick-borne diseases
2. Biomarkers in Animals Parasitology Research Group, Chulalongkorn University, Bangkok 10330, Thailand.
3. Parasitology Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Background and Aim: Canine tick-borne diseases are important diseases with a worldwide distribution. In Thailand, the most important canine tick-borne diseases are ehrlichiosis, babesiosis, and hepatozoonosis. This study aimed to determine the serum protein electrophoresis patterns (SPEPs) and C-reactive protein (CRP) levels associated with Ehrlichia canis, Babesia canis, or Hepatozoon canis single infections.
Materials and Methods: A total of 650 canine blood samples were collected from animal hospitals and clinics in Bangkok and its vicinity to examine health status and blood parasite infection. Suspected blood parasite infections were examined by buffy coat thin blood smear and confirmed by polymerase chain reaction. Normal dog and positive E. canis, B. canis, and H. canis single infections and serum protein profiles were determined by agarose gel electrophoresis. CRP concentration was measured by fluorescent immunoassay.
Results: In dogs infected with E. canis, B. canis, and H. canis single infections, albumin levels and A/G ratios significantly decreased, whereas β2-globulin levels increased (p<0.05). The γ-globulin level significantly increased in E. canis and H. canis infections (p<0.05). A monoclonal gammopathy pattern was observed in E. canis and B. canis single infections, whereas β-γ bridging patterns and increased β- and γ-globulin fractions were found in H. canis single infections. The CRP level increased in dogs with blood parasite single infections and may be related to the pathogenesis of the infection.
Conclusion: SPEPs and CRP levels can be used to monitor health status and blood parasite problems in infected dogs. Keywords: blood parasites, C-reactive protein, dog, electrophoresis, serum protein.
Keywords: blood parasites, C-reactive protein, dog, electrophoresis, serum protein.
How to cite this article: Asawakarn S, Taweethavonsawat P (2021) Characterization of serum protein electrophoresis patterns and C-reactive protein in canine tick-borne diseases, Veterinary World, 14(8): 2150-2154.
Received: 01-04-2021 Accepted: 19-07-2021 Published online: 21-08-2021
Corresponding author: Piyanan Taweethavonsawat E-mail: piyanan.t@chula.ac.th
DOI: 10.14202/vetworld.2021.2150-2154
Copyright: Asawakarn and Taweethavonsawat, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.