Apigenin induces oxidative stress in mouse Sertoli TM4 cells

Vet World Vol.14 December-2021 Article-9

Research Article

Veterinary World, 14(12): 3132-3137

https://doi.org/10.14202/vetworld.2021.3132-3137

Sathaporn Jittapalapong, Thapanee Poompoung, and Samak Sutjarit

Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.

Background and Aim: Apigenin (API) is an estrogenic compound found in many plants. Sertoli cells reside in the testis and are a key target of environmental toxicants. This study aimed to examine the cytotoxicity, especially oxidative stress of API in mouse Sertoli TM4 cells.

Materials and Methods: Mouse Sertoli TM4 cells were treated with 50 and 100 μM API for 48 h. Cell viability, lactate dehydrogenase (LDH) activities, glutathione reductase (GR) activities, production of reactive oxygen species (ROS), and malondialdehyde (MDA) levels were evaluated using various assays.

Results: Treatment with API at both 50 and 100 μM decreased viability and GR activity but increased LDH activity, ROS production, and MDA levels in mouse Sertoli TM4 cells.

Conclusion: Exposure to API induced oxidative stress in mouse Sertoli TM4 cells. Keywords: apigenin, malondialdehyde, oxidative stress, reactive oxygen species, TM4 cells.

Keywords: apigenin, malondialdehyde, oxidative stress, reactive oxygen species, TM4 cells.

How to cite this article: Jittapalapong S, Poompoung T, Sutjarit S (2021) Apigenin induces oxidative stress in mouse Sertoli TM4 cells, Veterinary World, 14(12): 3132-3137.

Received: 04-08-2021 Accepted: 02-11-2021 Published online: 20-12-2021

Corresponding author: Samak Sutjarit E-mail: cvtsms@ku.ac.th

DOI: 10.14202/vetworld.2021.3132-3137

Copyright: Jittapalapong, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.