Vet World Vol.14 January-2021 Article-37
Research Article
Veterinary World, 14(1): 285-291
https://doi.org/10.14202/vetworld.2021.285-291
Randomly amplified polymorphic DNA analysis of Staphylococcus chromogenes isolated from bovine and bubaline mastitis in Karnataka
2. Bioinformatics Centre, Department of Aquatic Animal Health Management, College of Fisheries, Mangaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Mangaluru, Karnataka, India.
3. Department of Microbiology, Veterinary College, Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.
4. Department of Pathology, Veterinary College, Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.
5. National Institute of Animal Biotechnology, Gachibowli, Hyderabad, Telangana, India.
Background and Aim: In recent times, non-aureus staphylococci (NAS) have emerged as the major organisms isolated from mastitis cases in dairy animals, with a predominance of Staphylococcus epidermidis and Staphylococcus chromogenes. As compared to Staphylococcus aureus, much less is known about the molecular types or the spatiotemporal epidemiology of these NAS species. In the present study, randomly amplified polymorphic DNA (RAPD) was employed to detect genetic polymorphisms, intraspecies diversity, and epidemiology of S. chromogenes strains (n=37) isolated from bovine and bubaline mastitis cases in the state of Karnataka.
Materials and Methods: Thirty-seven S. chromogenes isolates (14 from bovines and 23 from bubaline) isolated from subclinical mastitis cases, from organized and unorganized sectors, were subjected to RAPD typing. Further, methicillin resistance was determined by cefoxitin disk diffusion method.
Results: The amplified DNA fragments ranged from 150 to 3000 base pairs and yielded several RAPD profiles. Further analysis using Digital Image Correlation Engine correlation coefficient and UPGMA method showed that the 37 isolates could be classified into 12 distinct RAPD types (A to L) at 62% similarity (D=0.889). Four of the most predominant RAPD types, B, A, C, and E, in that order, and together, represented 65% of the isolates. High diversity was observed among the isolates both within farms and between geographic locations. Most of the isolates exhibited methicillin resistance. This is the first such report from India.
Conclusion: In the absence of defined multilocus sequence type protocols or sufficient sequences available in the public domain, RAPD can be employed to determine genetic diversity of S. chromogenes isolates. Keywords: mastitis, methicillin resistance, non-aureus staphylococci, randomly amplified polymorphic DNA, Staphylococcus chromogenes.
Keywords: mastitis, methicillin resistance, non-aureus staphylococci, randomly amplified polymorphic DNA, Staphylococcus chromogenes.
How to cite this article: Sheela P, Shekar M, Isloor S, Rathnamma D, Veeregowda BM, Satyanarayana ML, Sundareshan S, Shambulingappa BE, Hegde NR (2021) Randomly amplified polymorphic DNA analysis of Staphylococcus chromogenes isolated from bovine and bubaline mastitis in Karnataka, Veterinary World, 14(1): 285-291.
Received: 13-07-2020 Accepted: 14-12-2020 Published online: 30-01-2021
Corresponding author: P. Sheela E-mail: psheelasundar@gmail.com
DOI: 10.14202/vetworld.2021.285-291
Copyright: Sheela, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.