Vet World Vol.14 July-2021 Article-10
Research Article
Veterinary World, 14(7): 1797-1803
https://doi.org/10.14202/vetworld.2021.1797-1803
Evaluation of the solubility of 11-keto-β-boswellic acid and its histological effect on the diabetic mice liver using a novel technique
2. Institute of Chemical Sciences, University of Swat KP, Pakistan.
3. Natural and Medical Sciences Research Center, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
4. School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
Background and Aim: The literature is scant on the effect of 11-keto-β-boswellic acid (KBA) on the liver of diabetes-induced mice. This study was designed to develop a rapid, sensitive, accurate, and inexpensive detection technique for evaluating the solubility of KBA obtained from the gum resin of Omani frankincense (Boswellia sacra) in the liver of streptozotocin-induced diabetic mice using Fourier transform infrared (FTIR) reflectance spectroscopy coupled with principal components analysis (PCA). It also aimed to investigate the effect of KBA on histological changes in the hepatocytes of diabetic mice.
Materials and Methods: Eighteen mice were assigned to the healthy control group, the diabetic control group, or the KBA-treated diabetic group. Liver tissue samples from all groups were scanned using an FTIR reflectance spectrophotometer in reflection mode. FTIR reflectance spectra were collected in the wavenumber range of 400-4000 cm-1 using an attenuated total reflectance apparatus.
Results: FTIR reflectance spectra were analyzed using PCA. The PCA score plot, which is an exploratory multivariate data set, revealed complete segregation among the three groups' liver samples based on changes in the variation of wavenumber position in the FTIR reflectance spectra, which indicated a clear effect of KBA solubility on treatments. Histological analysis showed an improvement in the liver tissues, with normal structures of hepatocytes exhibiting mild vacuolation in their cytoplasm.
Conclusion: KBA improved the morphology of liver tissues in the diabetic mice and led to complete recovery of the damage observed in the diabetic control group. FTIR reflectance spectroscopy coupled with PCA could be deployed as a rapid, low-cost, and non-destructive detection method for evaluating treatment effects in diseased liver tissue based on the solubility of KBA. Keywords: 11-keto-β-boswellic acid, Fourier transform infrared reflectance, histology, liver, mice, principle component analysis.
Keywords: 11-keto-β-boswellic acid, Fourier transform infrared reflectance, histology, liver, mice, principle component analysis.
How to cite this article: Al Amri I, Mabood F, Kadim IT, Alkindi A, Al-Harrasi A, Al-Hashmi S, Abbas G, Hamaed A, Ahmed B, Al-Shuhaimi J, Khalaf S, Shaikh J (2021) Evaluation of the solubility of 11-keto-β-boswellic acid and its histological effect on the diabetic mice liver using a novel technique, Veterinary World, 14(7): 1797-1803.
Received: 31-01-2021 Accepted: 17-05-2021 Published online: 12-07-2021
Corresponding author: Isam T. Kadim E-mail: isam@unizwa.edu.om
DOI: 10.14202/vetworld.2021.1797-1803
Copyright: Al Amri, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.