Vet World Vol.14 July-2021 Article-9
Research Article
Veterinary World, 14(7): 1788-1796
https://doi.org/10.14202/vetworld.2021.1788-1796
Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models
2. Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
3. Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Brawijaya Malang, Jl. Mayjen Prof. Dr. Moestopo No. 47, Surabaya 60132, Indonesia.
Background and Aim: Endometriosis affects the ovaries and causes a decrease in the oocyte quality during endometrial receptivity. During the development of ovarian follicles, paracrine communication occurs between granulosa cells and oocytes. This study was conducted to determine the effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha (TNF-α) receptor 1 (TNFR1) expression, granulosa cell apoptosis, and folliculogenesis in endometriosis mouse models.
Materials and Methods: This study involved 42 female mice, which were divided into three groups: Healthy mice (T0), endometriosis mice without transplantation (T1), and endometriosis mice with bone marrow mesenchymal stem cell transplantation (T2). The mice were injected intraperitoneally with endometrial fragments (200 μL) to become endometriosis models. On day 15, the endometriosis models received mesenchymal stem cells. Sample collection was performed on day 29. Granulosa cell apoptosis and TNFR1 expression were examined using immunohistochemical staining, and folliculogenesis was assessed using hematoxylin and eosin staining of ovary samples. The data obtained from both examinations were statistically analyzed using Statistical Package for the Social Sciences.
Results: The results showed that TNFR1 expression is significantly decreased in T2 (p<0.004). The apoptosis of granulosa cells was lower in T2 (p<0.000). The primary, secondary, and graafian follicle counts in T2 were significantly increased.
Conclusion: Bone marrow mesenchymal stem cell transplantation in endometriosis mouse models can reduce TNFR1 expression and granulosa cell apoptosis and improve folliculogenesis. Keywords: apoptosis, bone marrow mesenchymal stem cells, endometriosis, folliculogenesis, granulosa cells, tumor necrosis factor-alpha receptor 1.
Keywords: apoptosis, bone marrow mesenchymal stem cells, endometriosis, folliculogenesis, granulosa cells, tumor necrosis factor-alpha receptor 1.
How to cite this article: Dwiningsih SR, Darmosoekarto S, Hendarto H, Dachlan EG, Rantam FA, Sunarjo S, Wiyasa IWA, Widjiati W (2021) Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models, Veterinary World, 14(7): 1788-1796.
Received: 25-01-2021 Accepted: 14-05-2021 Published online: 10-07-2021
Corresponding author: Widjiati Widjiati E-mail: widjiati@fkh.unair.ac.id
DOI: 10.14202/vetworld.2021.1788-1796
Copyright: Dwiningsih, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.