Vet World Vol.14 June-2021 Article-38
Research Article
Veterinary World, 14(6): 1711-1717
https://doi.org/10.14202/vetworld.2021.1711-1717
Changes in tear protein profile in dogs with keratoconjunctivitis sicca following topical treatment using cyclosporine A
2. Institute of Molecular Biosciences, Mahidol University, Salaya campus, Nakhonpathom, Thailand.
3. Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
4. Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
Background and Aim: Keratoconjunctivitis sicca (KCS) is a chronic inflammatory ocular disease that occurs in many dog breeds worldwide. This study aimed to investigate the tear protein pattern of healthy dogs, KCS dogs, and KCS dogs after treatment with cyclosporine A (CsA).
Materials and Methods: Twenty-eight dogs of any breed were enrolled in the study. The subjects were divided into three groups: Healthy, KCS, and CsA-treated dogs. Tear samples were collected using Schirmer strips. Tear proteins extracted from the strips were analyzed using two-dimensional electrophoresis. For the first dimension, total protein from tears was separated by isoelectric focusing. The second dimension was performed using 12.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The gel images were analyzed and the protein spots of differential expression were manually cut for protein annotation using mass spectrometry.
Results: In total, 12 protein spots were excised and subjected to protein identification. Associated with KCS, six protein spots were a downregulated protein, namely, lysozyme. The other six protein spots were upregulated in KCS dogs, consisting of heat shock protein beta-1, protein S100-A12, and keratin type II cytoskeletal 1 and 5. After treatment with CsA for 45 days, the lysozyme protein was still decreasing and the inflammation protein (S100-A12) was not identified.
Conclusion: Inflammatory tear proteins and proteins involved in cellular stress were present in KCS dogs and appeared to be reduced in medicated eyes. Treatment with topical CsA in the short term may not improve the activity of antibacterial proteins. Changes in the expression patterns of these four proteins might be useful for disease severity and progression assessment, as well as for exploring a novel method for dry eye management in dogs. Keywords: cyclosporine, dog, keratoconjunctivitis sicca, proteomic, tear protein.
Keywords: cyclosporine, dog, keratoconjunctivitis sicca, proteomic, tear protein.
How to cite this article: Sussadee M, Rucksaken R, Havanapan P, Reamtong O, Thayananuphat A (2021) Changes in tear protein profile in dogs with keratoconjunctivitis sicca following topical treatment using cyclosporine A, Veterinary World, 14(6): 1711-1717.
Received: 13-01-2021 Accepted: 12-05-2021 Published online: 30-06-2021
Corresponding author: Aree Thayananuphat E-mail: areethaya@gmail.com
DOI: 10.14202/vetworld.2021.1711-1717
Copyright: Sussadee, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.