Vet World Vol.14 March-2021 Article-3
Research Article
Veterinary World, 14(3): 569-577
https://doi.org/10.14202/vetworld.2021.569-577
Histopathological evaluation of Senecio rhizomatus Rusby in 7,12-dimethylbenz(α) anthracene-induced breast cancer in female rats
2. Department of Pharmacology, Bromatology and Toxicology, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr Puno 1002, Lima 15001, Peru.
3. Association for the Development of Student Research in Health Sciences, Faculty of Medicine. Universidad Nacional Mayor de San Marcos, Av. Miguel Grau 755, Cercado de Lima 15001, Peru.
4. Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja, San Cayetano s/n, 1101608 Loja, Ecuador.
5. Department of Pharmacology, Chitkara College of Pharmacy, Chitkara University, Punjab 140401, India.
Background and Aim: Senecio rhizomatus Rusby (SrR) is a medicinal plant of the Asteraceae family and traditionally consumed as infusion in the Andean region from Peru for inflammatory disorders. This study aimed to determine the histopathological changes afforded by SrR in 7, 12-dimethylbenz[α]anthracene (DMBA)-induced breast cancer (BC) in rats.
Materials and Methods: An ethanolic extract of SrR aerial parts was prepared by maceration with 96% ethanol, and the chemical components were identified by gas chromatography coupled to mass spectrometry; the antioxidant activity was determined by 1,1-diphenyl-2-picril-hidrazil (DPPH) assay; and the acute toxicity was assessed according to the OCED 423 guidelines. In a pharmacological study, 30 female Holztman rats were distributed randomly into five groups, as follows. Group I: Negative control (physiological serum, 2 mL/kg); Group II. DMBA (80 mg/Kg body weight); and Groups III, IV, and V: DMBA + ethanol extract of SrR at doses of 10, 100, and 200 mg/kg, respectively.
Results: The antioxidant activity of the SrR extract against DPPH was 92.50% at 200 μg/mL. The oral administration of SrR at doses of 50, 300, 2000, and 5000 mg/kg did not show any clinical evidence of toxicity or occurrence of death. The groups that received SrR presented a lower frequency of tumors and a cumulative tumor volume compared with the DMBA group (p<0.05); the DMBA group exhibited a higher incidence of necrosis and moderate mitosis, up to 66.67% and 100.00%, respectively. Finally, infiltrating carcinoma with extensive tumor necrosis was evidenced.
Conclusion: In experimental conditions, the ethanolic extract of SrR had a protective effect in DMBA-induced BC in female rats. Furthermore, the antioxidant activity of its main phytochemicals could be responsible for the effect observed, and SrR seems to be a safe extract in the preclinical phase. Keywords: breast cancer, carcinogenic, experimental pharmacology, phytotherapy, Senecio rhizomatus.
Keywords: breast cancer, carcinogenic, experimental pharmacology, phytotherapy, Senecio rhizomatus.
How to cite this article: Arroyo-Acevedo JL, Herrera-Calderon O, Rojas-Armas JP, Chávez-Asmat R, Calva J, Behl T (2021) Histopathological evaluation of Senecio rhizomatus Rusby in 7,12-dimethylbenz(α) anthracene-induced breast cancer in female rats, Veterinary World, 14(3): 569-577.
Received: 08-10-2020 Accepted: 25-01-2021 Published online: 06-03-2021
Corresponding author: Oscar Herrera-Calderon E-mail: oherreraca@unmsm.edu.pe
DOI: 10.14202/vetworld.2021.569-577
Copyright: Arroyo-Acevedo, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.