Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus)

Vet World Vol.14 March-2021 Article-37

Research Article

Veterinary World, 14(3): 820-824

https://doi.org/10.14202/vetworld.2021.820-824

Aulaani'am Aulanni'am¹, Krismal Marchel Ora², Nisa Ain Ariandini², Dyah Kinasih Wuragil³, Fajar Shodiq Permata⁴, Wibi Riawan⁵, and Ma Asuncion Guiang Beltran⁶

1. Biochemistry Laboratory, Faculty of Sciences, Brawijaya University, Indonesia.
2. Bachelor of Veterinary Student, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.
3. Laboratory of Veterinary Biochemistry, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.
4. Laboratory of Veterinary Histology, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.
5. Department of Molecular and Biochemistry, Faculty of Medicine, Brawijaya University, Indonesia.
6. Department of Microbiology and Veterinary Public Health, College of Veterinary Medicine, Tarlac Agricultural University, The Philippines.

Background and Aim: Gliricidia sepium is a medium-sized leguminous plant found widely in tropical to subtropical areas. It has been used as a medicinal ingredient and in rodenticides by local communities in both Indonesia and the Philippines. This study aimed to investigate the wound healing effects of an ointment containing G. sepium leaves on inflammatory cells using a rat model. We also determined its effect on the expression of interleukin (IL) 6 and IL-1β.

Materials and Methods: We used 16 Wistar male rats aged approximately 2 months and weighing 150-200 g. They were divided into four treatment groups (T1, positive control; T2, negative control; T3, wounds treated with G. sepium from Indonesia; and T4, wounds treated with G. sepium from the Philippines), and the ointment therapies were applied to wounds for 3 days. Hematoxylin and eosin staining was performed to examine the inflammatory cells microscopically. IL-1β and IL-6 expression were observed immunohistochemically.

Results: G. sepium leaves significantly (p<0.05) decreased the number of inflammatory cells, and the expression of IL-1β and IL-6 in the group treated with Indonesian G. sepium leaves was higher than that in the group treated with G. sepium leaves from the Philippines. The leaves contain flavonoids, saponins, and tannins, which act as anti-inflammatory agents to enhance the wound healing process.

Conclusion: Our findings suggest that G. sepium leaves from both the Philippines and Indonesia possess wound healing properties. Keywords: flavonoid, Gliricidia sepium leaves, herbal plants, saponin, tannin, wound healing.

Keywords: flavonoid, Gliricidia sepium leaves, herbal plants, saponin, tannin, wound healing.

How to cite this article: Aulanni'am A, Ora KM, Ariandini NA, Wuragil DK, Permata FS, Riawan W, Beltran MAG (2021) Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus), Veterinary World, 14(3): 820-824.

Received: 07-10-2020 Accepted: 04-02-2021 Published online: 31-03-2021

Corresponding author: Aulaani'am Aulanni'am E-mail: aulani@ub.ac.id

DOI: 10.14202/vetworld.2021.820-824

Copyright: Aulanni'am, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.