Vet World   Vol.14   March-2021  Article-8

Prevalence and molecular characterization of canine parvovirus

Background and Aim: Canine parvovirus (CPV) belonging to family Parvoviridae causes hemorrhagic gastroenteritis in dogs and heavy mortality in young dogs. The virus has three structural (VP1, VP2 and VP3) and two non-structural proteins (NS1 and NS2), VP2 being highly immunogenic. This study aims to study molecular epidemiology of CPV by sequence analysis of VP2 gene to determine the prevailing antigenic type(s) in the northern regions of India.

Materials and Methods: A total of 118 rectal swabs collected from dogs exhibiting clinical signs of CPV infection were processed for the isolation of DNA and subjected to polymerase chain reaction (PCR) and nested PCR (NPCR). A total of 13 NPCR products selected randomly were subjected to sequence analysis of VP2 gene.

Results: The percent positivity of CPV was found 28% and 70% by PCR and NPCR, respectively. Dogs with vaccination history against CPV too were found positive with a percent positivity of 24.10%. Gene sequencing and phylogenetic analysis of VP2 gene from these isolates revealed that most samples formed a clade with CPV-2a isolates.

Conclusion: Sequence analysis and phylogenetic analysis of VP2 gene in the studied regions of northern India revealed that CPV-2a was the most prevalent antigenic type. Keywords: canine parvovirus, nested polymerase chain reaction, polymerase chain reaction, sequence analysis, VP2 gene.

Keywords: canine parvovirus, nested polymerase chain reaction, polymerase chain reaction, sequence analysis, VP2 gene.

How to cite this article: Singh P, Kaur G, Chandra M, Dwivedi PN (2021) Prevalence and molecular characterization of canine parvovirus, Veterinary World, 14(3): 603-606.

Received: 18-08-2020  Accepted: 21-01-2021     Published online: 09-03-2021

Corresponding author: Gurpreet Kaur   E-mail: gurpreet7502@rediffmail.com

DOI: 10.14202/vetworld.2021.603-606

Copyright: Singh, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.