Vet World Vol.14 November-2021 Article-16
Research Article
Veterinary World, 14(11): 2957-2963
https://doi.org/10.14202/vetworld.2021.2957-2963
Inactivated vaccine against Aujeszky's disease
2. Laboratory of Microbiology , Research Institute for Biological Safety Problems, Gwardeiski, Kazakhstan.
3. Bacteriology Laboratory , Kazakh Scientific Research Veterinary Institute, Astana, Kazakhstan.
4. Department of Clinical Veterinary Medicine , Kazakh National Agrarian University, Almaty, Kazakhstan.
5. Laboratory o the diseases of domestic animals, Kyrgyz Research Institute of Veterinary named after A. Duysheev, Bishkek, Kyrgyzstan.
6. Laboratory of Diagnosis of Infectious Diseases, National Reference Veterinary Center Almaty Branch, Almaty, Kazakhstan.
Background and Aim: The Aujeszky's disease, also known as Pseudorabies, remains one of the most problematic fulminant diseases in domestic animals, affecting the central nervous system. The study aimed to investigate the effect of an inactivated vaccine against Aujeszky's disease based on "Kordai" virus strain.
Materials and Methods: To test the inactivation of the "Kordai" strain (grown by the roller method in VNK-21/13 cell culture with an infectious titer of at least 7.5 lg TCD50/ml) which is causative of Aujeszky's disease, next-generation teotropin and propolis preparations were used in concentrations of 0.1%, 0.08%, and 0.04%.
Results: As a result of comparative studies on the optimization of parameters for inactivating the "Kordai" virus strain, it was established that teotropin is a more effective inactivant than propolis. At the same time, the optimal final concentration of teotropin for inactivation was 0.1%, along with a reaction medium temperature of 37°C, pH of 7.4-7.6, and duration of inactivation of 14 h. The titer of virus-neutralizing activity (VNA) of antibodies at the pH (neutralization reactions) in vaccinated sheep of 10-12 months of age was 7.5±0.3, Ig TCID50/ml (tissue culture infectious dose 50%), and 3.5±0.3 in the cell culture VNK-21/13 (culture of Syrian hamster kidney cells).
Conclusion: To determine colostral immunity in newborn lambs, the method of metabolic status correction was used to vaccinate lambs obtained from immune sheep 4 months after birth. The results showed that lambs obtained from immune sheep had high VNA titers. A sustained immune response in vaccinated animals was obtained after double vaccination. Keywords: adjuvant, Aujeszky's disease virus, colostral immunity, immunogenicity, strain "Kordai."
Keywords: adjuvant, Aujeszky's disease virus, colostral immunity, immunogenicity, strain "Kordai."
How to cite this article: Kondibaeva ZB, Yespembetov BA, Abeuov KB, Mussayeva AK, Siyabekov ST, Nussupova ST, Akmatova EK, Pazylov YK, Maikhin KT, Syrym NS (2021) Inactivated vaccine against Aujeszky's disease, Veterinary World, 14(11): 2957-2963.
Received: 25-05-2021 Accepted: 21-09-2021 Published online: 24-11-2021
Corresponding author: Nazym S. Syrym E-mail: nazym_syrym@rambler.ru
DOI: 10.14202/vetworld.2021.2957-2963
Copyright: Kondibaeva, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.