Vet World Vol.14 October-2021 Article-9
Research Article
Veterinary World, 14(10): 2640-2645
https://doi.org/10.14202/vetworld.2021.2640-2645
In ovo inhibition of avian pox virus replication by mangosteen rind and red ginger ethanolic extracts
2. Department of Internal Medicine, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
3. Department of Microbiology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
Background and Aim: Avian pox is a contagious disease caused by the avian pox virus (APV). Mangostin and γ-mangostin in mangosteen rind (MR) and gingerol in red ginger (RG) exhibit antiviral activity. In this study, we evaluated the effect of MR and RG ethanolic extracts on APV based on pock lesions on the chorioallantoic membrane (CAM) of specific pathogen-free (SPF) embryonated chicken eggs (ECEs).
Materials and Methods: Three APVs from chicken isolates (C1, C2, and C3), one APV from a pigeon isolate (P), 1.5% and 3% MR ethanolic extract, 5% and 10% RG ethanolic extract, and a combination of 1.5% MR and 5% RG at 0.1 mL/ egg were inoculated in ovo (7th day incubation, chorioallantoic route) in SPF ECEs. A control group inoculated in ovo with APV alone was also established. Each treatment consisted of three replicates. Parameters including embryo survival, CAM lesions, and average number of pock lesions were determined.
Results: In ovo inoculation of MR and RG ethanolic extracts was not harmful to the ECEs and did not induce CAM lesions. The average number of pock lesions in the control group (C1, C2, C3, and P) was 35, 14, 10, and 17, respectively, whereas in all treatment groups, the number was 0, except in the 5% RG group of C1, which had a value of 10.
Conclusion: In ovo inoculation of 1.5% and 3% MR, 5% and 10% RG, and the combination of 1.5% MR plus 5% RG ethanolic extract s at 0.1 mL/egg inhibit APV by reducing the number of pock lesions on the CAM of the ECE. Keywords: avian pox virus, in ovo, mangosteen rind, pock lesions, red ginger.
Keywords: avian pox virus, in ovo, mangosteen rind, pock lesions, red ginger.
How to cite this article: Nur'aini AL, Hartati S, Untari T (2021) In ovo inhibition of avian pox virus replication by mangosteen rind and red ginger ethanolic extract s, Veterinary World, 14(10): 2640-2645.
Received: 27-04-2021 Accepted: 27-08-2021 Published online: 11-10-2021
Corresponding author: Sri Hartati E-mail: sri_hartati@ugm.ac.id
DOI: 10.14202/vetworld.2021.2640-2645
Copyright: Nur'aini, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.