Follicle-stimulating hormone mediates the consumption of serum-derived glycogen by bovine cumulus-oocyte complexes during in vitro maturation

Vet World Vol.14 September-2021 Article-27

Research Article

Veterinary World, 14(9): 2512-2517

https://doi.org/10.14202/vetworld.2021.2512-2517

Ludymila F. Cantanhêde¹, Cristiane T. Santos-Silva², Marcelo T. Moura¹, José C. Ferreira-Silva¹, Júnior M. B. Oliveira¹, Daniel N. A. Gonçalves¹, Álvaro A. C. Teixeira², Valéria Wanderley-Teixeira², and Marcos A. L. Oliveira¹

1. Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife 52171900, Brazil.
2. Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco - UFRPE, Recife 52171900, Brazil.

Background and Aim: Oocyte in vitro maturation (IVM) is an appealing approach for several assisted reproductive technologies and dissecting oocyte maturation. Nonetheless, IVM leads to lower developmental competence and usually relies on undefined, serum-containing media. Therefore, biochemical profiling aimed to explore fluctuations in IVM media content during the acquisition of oocyte developmental competence.

Materials and Methods: Bovine cumulus-oocyte complexes (COCs) underwent IVM in TCM199 medium with Earle's salts, supplemented with 2.0 mM L-glutamine, 10% fetal bovine serum, antibiotics, and 0.05 IU/mL porcine follicle-stimulating hormone (FSH+) or vehicle control (CTL) medium for 22 h.

Results: FSH withdrawal (CTL) diminished several processes associated with the acquisition of oocyte developmental competence, such as reduced cumulus cell expansion, diminished estradiol synthesis (FSH+: 116.0±0.0 pg/mL vs. CTL: 97.6±18.0 pg/mL), and lower oocyte nuclear maturation rate (FSH+: 96.47% vs. CTL: 88.76%). Fresh media formulations (i.e., TCM199 with FSH or vehicle) were indistinguishable under biochemical profiling threshold conditions. Biochemical profiling showed similar total protein and lipid concentrations between groups. Further, total sugar concentrations diminished from fresh media to their post-IVM counterparts, albeit in an FSH-independent manner. Glycogen concentrations remained unaltered after IVM within CTL media, albeit were substantially lower after IVM under FSH+ conditions.

Conclusion: FSH mediates the consumption of serum-derived glycogen by bovine COCs during IVM and implies that serum-free media should contain increased glucose concentrations to facilitate the acquisition of oocyte developmental competence. Keywords: biomolecules, cattle, developmental competence, gonadotropin, granulosa, sera.

Keywords: biomolecules, cattle, developmental competence, gonadotropin, granulosa, sera.

How to cite this article: Cantanhêde LF, Santos-Silva CT, Moura MT, Ferreira-Silva JC, Oliveira JMB, Gonçalves DNA, Teixeira ÁAC, Wanderley-Teixeira V, Oliveira MAL (2021) Follicle-stimulating hormone mediates the consumption of serum-derived glycogen by bovine cumulus-oocyte complexes during in vitro maturation, Veterinary World, 14(9): 2512-2517.

Received: 09-05-2021 Accepted: 23-08-2021 Published online: 24-09-2021

Corresponding author: Marcelo T. Moura E-mail: marcelotmoura@gmail.com

DOI: 10.14202/vetworld.2021.2512-2517

Copyright: Cantanhêde, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.