Vet World Vol.14 September-2021 Article-3
Research Article
Veterinary World, 14(9): 2313-2316
https://doi.org/10.14202/vetworld.2021.2313-2316
Screening of in vitro antimicrobial effects of Helicteres isora extract against Staphylococcus aureus
2. A.T. Animal Clinic 284/3 Village No. 13, Kaset Wisai Roi Et, Thailand.
Background and Aim: Staphylococcus aureus is an important pathogen causing contagious mastitis in cows that need novel treatment rather than antibiotic therapy. This study aimed to investigate the antimicrobial activity of Helicteres isora extracts against S. aureus isolated from subclinical and clinical mastitis cows using an in vitro model.
Materials and Methods: H. isora pods were extracted using the following solvents: Distilled water, ethanol, acetone, and methanol. The antimicrobial activity of each extract was determined by the disk diffusion method and broth microdilution assay to assess the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).
Results: The ethanolic extract of H. isora exhibited the largest inhibition zones against S. aureus (31.05±1.20 mm), followed by the aqueous, methanolic, and acetone extracts, respectively (26.34±1.15, 24.23±0.50, and 22.46±1.53). The ethanolic extract also had a strong inhibitory effect on S. aureus, with MIC and MBC of 0.13 and 0.52 mg/mL, respectively.
Conclusion: This study revealed that H. isora is a potential alternative natural antibacterial agent against S. aureus infection. The antimicrobial activity of H. isora is most likely mediated by phytochemical constituents. Keywords: antimicrobial, Helicteres isora, herbs, mastitis, Staphylococcus aureus.
Keywords: antimicrobial, Helicteres isora, herbs, mastitis, Staphylococcus aureus.
How to cite this article: Sirimongkolvorakul S, Jasancheun A (2021) Screening of in vitro antimicrobial effects of Helicteres isora extract against Staphylococcus aureus, Veterinary World, 14(9): 2313-2316.
Received: 15-03-2021 Accepted: 30-07-2021 Published online: 04-09-2021
Corresponding author: Sunisa Sirimongkolvorakul E-mail: sirikul_sunii@yahoo.com
DOI: 10.14202/vetworld.2021.2313-2316
Copyright: Sirimongkolvorakul and Jasancheun, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.