Vet World   Vol.15   April-2022  Article-1

Research Article

Veterinary World, 15(4): 818-826

https://doi.org/10.14202/vetworld.2022.818-826

Growth performance, blood variables, intestinal bacterial content, and morphological measurements of broilers supplemented with Lactobacillus casei-fermented mixture of red rice and aromatic ginger

Fitri Dwi Astuti1, Sugiharto Sugiharto1, Turrini Yudiarti1, Endang Widiastuti1, Hanny I. Wahyuni1, and Tugay Ayaşan2
1. Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, Semarang, Central Java, Indonesia.
2. Osmaniye Korkut Ata University, Kadirli Faculty of Applied Sciences, Department of Organic Farming Business Management, Osmaniye, Turkey.

Background and Aim: Natural feed additives are important for broiler sustainability during the post-antibiotic era. This study aimed to evaluate the possible effects of the dietary supplementation of Lactobacillus casei-fermented mixture of red rice and aromatic ginger (FERMIX) on growth, blood profiles, intestinal bacterial content, and morphological measurements of broilers.

Materials and Methods: Two hundred broiler chicks were allotted to four groups with five replications, including CONTROL (chicks provided with basal feed), FERMIX025 (basal feed supplemented with 0.25% FERMIX), FERMIX050 (0.50% FERMIX), and FERMIX100 (1.00% FERMIX). FERMIX is an anaerobic fermentation product from a mixture of red rice flour and aromatic ginger using L. casei. Blood, organs, digesta, and carcass were collected on day 35.

Results: Final body weight, weight gain, feed intake, and feed conversion ratio did not differ (p>0.05) across treatments. FERMIX at 0.5% increased (p<0.05) spleen relative weight. Liver weight was lower (p<0.05) in broilers fed with 1% FERMIX. The liver weight linearly reduced (p<0.05) with the increased FERMIX levels. FERMIX at 0.25% elevated (p<0.05) broiler drumsticks' yield than that in other groups. Erythrocytes, hemoglobin, packed cell volume, and plasma total protein levels were quadratically higher (p<0.05) in FERMIX050 treated than in other groups. Increased FERMIX levels resulted in a linear decrease (p=0.08) in ileal lactose-negative Enterobacteriaceae counts. Villi height/crypt depth (CD) ratio was quadratically higher (p<0.05) in the duodenum of broiler supplemented with 0.5% FERMIX. The jejunal CD was quadratically lower (p<0.05) in FERMIX050 than in other groups.

Conclusion: Although it did not influence growth, dietary FERMIX, especially at 0.5%, improved immune competencies, physiological conditions, and health of broilers' intestine. Keywords: broiler, fermentation, health, phytogenic, prebiotic, probiotic.

Keywords: broiler, fermentation, health, phytogenic, prebiotic, probiotic.

How to cite this article: Astuti FD, Sugiharto S, Yudiarti T, Widiastuti E, Wahyuni HI, Ayaşan T (2022) Growth performance, blood variables, intestinal bacterial content, and morphological measurements of broilers supplemented with Lactobacillus casei-fermented mixture of red rice and aromatic ginger, Veterinary World, 15(4): 818-826.

Received: 29-11-2021  Accepted: 23-02-2022     Published online: 02-04-2022

Corresponding author: Sugiharto Sugiharto   E-mail: sgh_undip@yahoo.co.id

DOI: 10.14202/vetworld.2022.818-826

Copyright: Astuti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.