Vet World Vol.15 April-2022 Article-5
Research Article
Veterinary World, 15(4): 848-854
https://doi.org/10.14202/vetworld.2022.848-854
Enhancement of the antifungal activity of some antimycotics by farnesol and reduction of Candida albicans pathogenicity in a quail model experiment
2. Department of Biochemistry, Institute of Medicine, Peoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia.
3. Department of Veterinary Medicine, Agrarian Technological Institute, Peoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia.
4. Department of General Pharmaceutical and Biomedical Technologies, Institute of Medicine, Peoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia.
5. Department of Foreign Languages, Institute of Medicine, Peoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia.
Background and Aim: Clinical strains of microorganisms, including pathogenic yeast-like fungi (YLF), are resistant to currently used antifungal agents. Thus, it is relevant to study the combinations of existing antimicrobial drugs and a medicinal extract of plant origin (farnesol). In previous studies, farnesol showed a relatively strong anti-biofilm effect against Candida albicans. This study aimed to determine how much the resistance profile of non-biofilm microorganisms can change.
Materials and Methods: Six clinical isolates of C. albicans and one reference strain were used to study the interaction of farnesol with the most used antimycotics. To determine the sensitivity of YLF to antimycotic drugs, such as nystatin (50 μg), amphotericin B (10 μg), ketoconazole (10 μg), clotrimazole (10 μg), voriconazole (10 μg), fluconazole (25 μg), miconazole (10 μg), and intraconazole (10 μg), the classic disk diffusion method was used. In the second stage, one of the six strains was used to simulate candidiasis of the gastrointestinal tract in an in vivo quail model. As an unusual experimental design, this study investigated the effects of pretreated C. albicans in quails, not the in vivo pathogenicity of C. albicans, after treatment with farnesol.
Results: The resistance profiles of Candida strains did not improve with farnesol in all strains. All concentrations of farnesol (100, 50, and 25 μM) demonstrated a fungistatic effect (i.e., an increase in drug sensitivity) in 23 of 56 (7×8) cases (41%). The remaining 54% demonstrated no changes in the resistance to antifungal drugs or deterioration of the indicators in rare cases (5%). At 100 μM farnesol, sensitivity improved in 33 of 56 cases (59%). Candidiasis or the severity of clinical disease of the quail digestive tract developed to a lesser extent if fungi were treated with farnesol.
Conclusion: Farnesol does not always show a positive result on single cells without biofilm in the laboratory. However, in a biofilm or an in vivo model with biofilms, farnesol can be considered a new antimycotic drug or an additive to existing antimycotics. Keywords: amphotericin B, antimycotics, Candida albicans, clotrimazole, farnesol, fluconazole, intraconazole, ketoconazole, miconazole, nystatin, quail model, quorum sensing, voriconazole.
Keywords: amphotericin B, antimycotics, Candida albicans, clotrimazole, farnesol, fluconazole, intraconazole, ketoconazole, miconazole, nystatin, quail model, quorum sensing, voriconazole.
How to cite this article: Sachivkina N, Senyagin A, Podoprigora I, Vasilieva E, Kuznetsova O, Karamyan A, Ibragimova A, Zhabo N, Molchanova M (2022) Enhancement of the antifungal activity of some antimycotics by farnesol and reduction of Candida albicans pathogenicity in a quail model experiment, Veterinary World, 15(4): 848-854.
Received: 20-11-2021 Accepted: 28-02-2022 Published online: 08-04-2022
Corresponding author: Nadezhda Sachivkina E-mail: sachivkina@yandex.ru
DOI: 10.14202/vetworld.2022.848-854
Copyright: Sachivkina, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.