Vet World Vol.15 April-2022 Article-9
Research Article
Veterinary World, 15(4): 885-889
https://doi.org/10.14202/vetworld.2022.885-889
Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide
2. Department of Animal Science, Kasetsart University, Bangkok 10900, Thailand.
3. Department of Livestock Development, Veterinary Research and Development Center (Upper Southern Region), Nakhon Si Thammarat 80110, Thailand.
4. Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand.
5. One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand. Food Technology and Innovation Center of Excellence, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
6. One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Background and Aim: The principal cytokines released by the host on infection include pro-inflammatory cytokines such as interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF). These cytokines were regarded as regulators of the host's response to infection. This study aimed to determine the release of pro-inflammatory cytokines from chicken peripheral blood mononuclear cells (PBMCs) following lipopolysaccharide (LPS) stimulation.
Materials and Methods: Blood samples were collected from six Betong chickens. To isolate PBMCs, density gradient centrifugation was utilized. PBMC culture in RPMI1640 with 10% fetal bovine serum was stimulated with various concentrations of LPS (0, 0.01, 0.1, and 1.0 μg/mL). The production of TNF-α, IL-1β, and IL-6 was determined using an enzyme-linked immunosorbent assay.
Results: When the PBMCs were cultured for 24 h with varying doses of LPS, there was no significant variation in cell viability. TNF-α, IL-1β, and IL-6 levels were measured in Betong chicken PBMC. The release of these cytokines increased considerably as LPS concentration (0.01-1 μg/mL) increased (p<0.05).
Conclusion: In vitro studies of the chicken immune response, notably the release of pro-inflammatory cytokines, can be conducted using PBMCs obtained from chicken blood. Keywords: chicken, interleukin-1β, interleukin-6, peripheral blood mononuclear cell, tumor necrosis factor-α.
Keywords: chicken, interleukin-1β, interleukin-6, peripheral blood mononuclear cell, tumor necrosis factor-α.
How to cite this article: Rattanasrisomporn J, Tantikositruj C, Thiptara A, Kitpipit W, Wichianrat I, Kayan A, Boonkaewwan C (2022) Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide, Veterinary World, 15(4): 885-889.
Received: 30-10-2021 Accepted: 15-02-2022 Published online: 11-04-2022
Corresponding author: Chaiwat Boonkaewwan E-mail: chaiwat.bo@wu.ac.th
DOI: 10.14202/vetworld.2022.885-889
Copyright: Rattanasrisomporn, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.