Vet World Vol.15 August-2022 Article-14
Research Article
Veterinary World, 15(8): 2012-2020
https://doi.org/10.14202/vetworld.2022.2012-2020
Long-term intake of Lilium lancifolium mitigated osteoarthritic effects by suppressing inflammatory cytokines in a dog model
2. Department of Veterinary Medicine and Surgery College of Veterinary Medicine, Sudan University of Science and Technology, Hilat Kuku, Khartoum 11311, Sudan.
3. Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan 54538, Republic of Korea.
Background and Aim: Osteoarthritis (OA) is a chronic, painful, degenerative inflammatory disease of the synovial joints. Regular use of nonsteroidal anti-inflammatory drugs to decrease OA pain can have severe side effects, such as gastric irritation, ulcers, and heart problems. Natural products are extensively used to minimize OA-associated pain and inflammatory reactions. Lilium lancifolium is commonly used to alleviate several diseases through its anti-inflammatory effects. This study examined the impact of L. lancifolium extract on alleviating pain and inflammation associated with articular cartilage damage.
Materials and Methods: Hydro-ethanol extracts of the L. lancifolium bulb were used. The experimental animals (adult beagle dogs) were divided into four groups: sham, which received neither treatment nor surgery; placebo, which received an empty gelatin capsule; glucosamine, which received glutamine (60 mg/kg); and L. lancifolium, which received an L. lancifolium extract-filled (60 mg/kg) gelatin capsule for 8 weeks. OA was induced by an expert orthopedic surgeon in 2-year-old dogs through resection of cranial cruciate ligament and lateral collateral ligament. Inflammatory cytokines, enzymes, lameness score, radiology, and histological changes were assessed.
Results: Our experiments showed that long-term oral therapy with L. lancifolium alleviated inflammation and increased histological damage. L. lancifolium treatment effectively reduced cytokines, such as interleukin-6, metalloproteinase-9, leukotriene-4, prostaglandin, and cyclo-oxygenase in dogs with OA, suggesting the potential to minimize inflammatory reactions in OA. L. lancifolium showed anti-inflammatory qualities in dogs with OA. This effect was comparable with that of glucosamine OA treatment.
Conclusion: L. lancifolium supplementation represents a possible therapeutic and management option in this model of OA. Keywords: articular cartilage, inflammation, joint pain, Lilium lancifolium, MMP-9, osteoarthritis.
Keywords: articular cartilage, inflammation, joint pain, Lilium lancifolium, MMP-9, osteoarthritis.
How to cite this article: Cho J, Park Y, Choi J, Adam GO, Ju E, Park H, and Oh H (2022) Long-term intake of Lilium lancifolium mitigated osteoarthritic effects by suppressing inflammatory cytokines in a dog model, Veterinary World, 15(8): 2012–2020.
Received: 12-04-2022 Accepted: 07-07-2022 Published online: 22-08-2022
Corresponding author: Hong-Geun Oh E-mail: dvmoh@hanmail.net
DOI: 10.14202/vetworld.2022.2012-2020
Copyright: Cho, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.