Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java, Indonesia

Vet World Vol.15 August-2022 Article-15

Research Article

Veterinary World, 15(8): 2021-2028

https://doi.org/10.14202/vetworld.2022.2021-2028

Wiwiek Tyasningsih¹, Sancaka Chasyer Ramandinianto², Ribby Ansharieta², Adiana Mutamsari Witaningrum³, Dian Ayu Permatasari³, Dhandy Koesoemo Wardhana³, Mustofa Helmi Effendi³, and Emmanuel Nnabuike Ugbo⁴

1. Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
2. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
3. Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
4. Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria.

Background and Aim: Raw milk can be a source of food-borne disease transmission and a medium for spreading antibiotic-resistant bacteria. Staphylococcus aureus and Escherichia coli are bacteria that have the pathogenic ability to attack host cells and are capable of harboring antibiotic-resistant genes. This study estimated the prevalence and antibiotic resistance of S. aureus and E. coli isolated from raw milk in East Java, Indonesia.

Materials and Methods: Two hundred and fifty raw milk samples were collected from five dairy farms in East Java. S. aureus and E. coli were isolated using their respective selective media, whereas antibiotic susceptibility testing was performed using the Kirby–Bauer disk diffusion method. The methicillin-resistant S. aureus (MRSA) was confirmed using the oxacillin resistance screen agar test, and extended-spectrum beta-lactamase (ESBL)-producing E. coli was determined using the double-disk synergy test. The presence of mecA and blaTEM genes were screened by the polymerase chain reaction method.

Results: Results indicated that the prevalence of S. aureus was 138 (55.2%) and that E. coli was 176 (70.4%). Of the 138 S. aureus isolated, 27 (19.6%) were MRSA, and among the 176 E. coli isolates identified, 3 (1.7%) were ESBL producers. The mecA gene was observed in 2 (7.4%) MRSA and all 3 (100%) ESBL-producing E. coli isolated harbored blaTEM genes.

Conclusion: The presence of MRSA and ESBL-producing E. coli in raw milk is a serious public health threat, and public awareness should be raised about the dangers posed by these pathogenic organisms. Keywords: Escherichia coli, extended-spectrum beta-lactamase, methicillin-resistant Staphylococcus aureus, public health, raw milk, Staphylococcus aureus.

Keywords: Escherichia coli, extended-spectrum beta-lactamase, methicillin-resistant Staphylococcus aureus, public health, raw milk, Staphylococcus aureus.

How to cite this article: Tyasningsih W, Ramandinianto SC, Ansharieta R, Witaningrum AM, Permatasari DA, Wardhana DK, Effendi MH, and Ugbo EN (2022) Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java, Indonesia, Veterinary World, 15(8): 2021–2028.

Received: 13-04-2022 Accepted: 11-07-2022 Published online: 23-08-2022

Corresponding author: Mustofa Helmi Effendi E-mail: mustofa-h-e@fkh.unair.ac.id

DOI: 10.14202/vetworld.2022.2021-2028

Copyright: Tyasningsih, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.