Vet World Vol.15 August-2022 Article-16
Research Article
Veterinary World, 15(8): 2029-2038
https://doi.org/10.14202/vetworld.2022.2029-2038
A first attempt at determining the antibody-specific pattern of Platynosomum fastosum crude antigen and identification of immunoreactive proteins for immunodiagnosis of feline platynosomiasis
2. Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
3. Department of Molecular Tropical Medicine and Genetics, Mahidol University, Bangkok, Thailand.
4. Parasitology Research Laboratory, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
5. Parasitology Unit, Department of Pathology, Feline Infectious Disease and Health for Excellence Research Unit, Animal Vector-Borne Disease Research Unit, Microbial Food Safety and Antimicrobial Resistance Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Background and Aim: Feline platynosomiasis, also known as lizard poisoning, is a feline hepatic disease caused by the parasitic trematode Platynosomum fastosum. Since this helminth resides in biliary ducts and gallbladder, the heavy infection can lead to failure of the hepatobiliary system and can be associated with cholangiocarcinoma. The primary diagnostic tool currently used is conventional fecal microscopy. However, low sensitivity of detection could occur in the case of light infection or biliary obstruction. This study aimed to determine the antibody-specific pattern of P. fastosum crude antigen and to identify immunoreactive proteins to develop the immunodiagnostic techniques.
Materials and Methods: We investigated potential antigens specific to P. fastosum infection using western blotting. Forty-six samples of cat serum, including 16 P. fastosum-infected sera, eight healthy control sera, and 22 sera infected with other endoparasites were used. The sensitivity, specificity, positive predictive value, and negative predictive value of each band were calculated. Immunoreactive bands with high diagnostic values were further analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify the protein components.
Results: Using immunoblotting, three proteins of 72 kDa, 53 kDa, and 13 kDa were found to be immunogenic. LC-MS/MS identified these proteins as a 70 kDa heat shock protein, a hypothetical protein (CRM22_002083) (adenosine triphosphate synthase subunit beta), and histone H2B, respectively.
Conclusion: This study is the first to reveal three proteins that could be candidates for developing diagnostic tools for feline platynosomiasis. Keywords: candidate antigens, crude worm extracts, immunoblotting, liquid chromatography-tandem mass spectrometry, Platynosomum fastosum.
Keywords: candidate antigens, crude worm extracts, immunoblotting, liquid chromatography-tandem mass spectrometry, Platynosomum fastosum.
How to cite this article: Soe BK, Adisakwattana P, Reamtong O, Anuracpreeda P, and Sukhumavasi W (2022) A first attempt at determining the antibody-specific pattern of Platynosomum fastosum crude antigen and identification of immunoreactive proteins for immunodiagnosis of feline platynosomiasis, Veterinary World, 15(8): 2029–2038.
Received: 17-03-2022 Accepted: 18-07-2022 Published online: 23-08-2022
Corresponding author: Woraporn Sukhumavasi E-mail: vetkwan@hotmail.com / woraporn.s@chula.ac.th
DOI: 10.14202/vetworld.2022.2029-2038
Copyright: Soe, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.