Vet World Vol.15 December-2022 Article-18
Research Article
Veterinary World, 15(12): 2896-2902
https://doi.org/10.14202/vetworld.2022.2896-2902
Toxicity test of flavonoid compounds from the leaves of Dendrophthoe pentandra (L.) Miq. using in vitro culture cell models
2. Department of Pharmaceutics, Faculty of Pharmacy, Universitas Airlangga, Mulyorejo Road, 60115, Surabaya, Indonesia.
3. Department of Veterinary Medicine, Asia University, No. 500, Lioufeng Road, Wufeng District, Taichung 41354, Taiwan.
Background and Aim: The flavonoids from mistletoe are thought to have antimicrobial action. This encouraging finding supports the benefits of medicinal plants as a substitute for synthetic antimicrobials, thus promoting healthy lifestyles. In contrast, it is known that the use of topical drug formulations made from flavonoids of mistletoe (Dendrophthoe pentandra (L.) Miq. Loranthaceae) with Indonesian name, Benalu duku (BD) is required in skin cell irritation. This study aimed to assess the toxic effects of the flavonoid substances of BD, as an initial screening.
Materials and Methods: A myeloma cell line was cultured in Roswell Park Memorial Institute medium, and the Baby Hamster Kidney clone 12 (BHK21) cell line was cultured in Dulbecco's Modified Eagle's Medium from stock (±9 × 107 cells/mL), and 1.2 mL of culture were distributed into each well of a microtiter plate. Subsequently, 0.2 mL of serially diluted flavonoid compounds (0.5–3 μg/mL) were added to 12 wells for each concentration, as trial groups (including control groups), followed by a 2-day incubation. Observations were performed based on the cytopathic effect (CPE) using an inverted microscope at a magnification of 100×.
Results: Cytopathic effect was detected on the microtiter plate wells for the groups of myeloma and BHK21 cells at a flavonoid concentration of 0.5 μg/mL–3 μg/mL.
Conclusion: Flavonoid compounds from BD were safely used for topical treatment of cancer cells at a concentration <2.491 μg/mL, whereas for non-cancerous cells, a concentration <2.582 μg/mL was sufficient (p < 0.05). Keywords: antimicrobial, Baby Hamster Kidney clone 12 cell culture, cytopathic effect, healthy lifestyle, myeloma cell culture.
Keywords: antimicrobial, Baby Hamster Kidney clone 12 cell culture, cytopathic effect, healthy lifestyle, myeloma cell culture.
How to cite this article: Lazuardi M, Suharjono S, Chien C, He J, Lee C, Peng C, Sukmanadi M, Sugihartuti R, and Maslachah L (2022) Toxicity test of flavonoid compounds from the leaves of Dendrophthoe pentandra (L.) Miq. using in vitro culture cell models, Veterinary World, 15(12): 2896–2902.
Received: 17-06-2022 Accepted: 09-11-2022 Published online: 21-12-2022
Corresponding author: Mochamad Lazuardi E-mail: lazuardi@fkh.unair.ac.id
DOI: 10.14202/vetworld.2022.2896-2902
Copyright: Lazuardi, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.