Extended-spectrum beta-lactamase-producing Escherichia coli from pork in Muang district, Chiang Mai Province, Thailand

Vet World Vol.15 December-2022 Article-19

Research Article

Veterinary World, 15(12): 2903-2909

https://doi.org/10.14202/vetworld.2022.2903-2909

Wirunchana Srichumporn¹, Warangkhana Chaisowwong², Montira Intanon², and Kannika Na-Lampang^2,3

1. Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
2. Department of Veterinary Bioscience and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
3. Center of Excellence in Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.

Background and Aim: Contaminated pork is one of the transmission routes for pathogens. Extended-spectrum beta-lactamases (ESBL)-producing Escherichia coli is one of the critical threats to global public health. This study aimed to examine pork from different types of markets in Muang district, Chiangmai Province, Thailand, for a proportion of ESBL-producing E. coli, antibiotic resistance of ESBL-producing E. coli and ESBL-producing E. coli genotypes.

Materials and Methods: Samples were collected from different market types; fresh markets, pork stores, and supermarkets, enriched and inoculated on selective media. Extended-spectrum beta-lactamases-producing E. coli was identified using double-disk diffusion method according to Clinical and Laboratory Standards Institute 2016. Antibiotic susceptibility test was performed through VITEK® System and ESBL-encoding genes were detected using a multiplex polymerase chain reaction.

Results: About 69% of the samples were positive to ESBL-producing E. coli and showed high rates of resistance for ampicillin (100%), piperacillin (100%), cefalexin (100%), cefpodoxime (100%), cefovecin (100%) and ceftiofur (100%), gentamycin (89.86%), and tetracycline (TE) (84.06%). All isolates were multiple drug resistant; resistance patterns of beta-lactams, aminoglycosides, TEs, nitrobenzene derivatives, and sulfonamide groups were observed. The ESBL-producing E. coli-positive isolates carried blaCTX-M groups (100%), blaTEM (98.55%), and blaSHV (1.45%). None of blaOXA was found in this study.

Conclusion: Extended-spectrum beta-lactamases-producing E. coli was found in various types of markets; all isolates were detected as multidrug-resistant. The dissemination of such strains can conceivably cause concerning public health, implying that supervised antimicrobial use in pork production and sanitary food preparation is recommended. Keywords: antimicrobial resistance, extended-spectrum beta-lactamases-producing Escherichia coli, pork, Thailand.

Keywords: antimicrobial resistance, extended-spectrum beta-lactamases-producing Escherichia coli, pork, Thailand.

How to cite this article: Srichumporn W, Chaisowwong W, Intanon M, and Na-Lampang K (2022) Extended-spectrum beta-lactamase-producing Escherichia coli from pork in Muang district, Chiang Mai Province, Thailand, Veterinary World, 15(12): 2903–2909.

Received: 14-06-2022 Accepted: 15-11-2022 Published online: 21-12-2022

Corresponding author: Kannika Na-Lampang E-mail: kannika.nalampang@cmu.ac.th

DOI: 10.14202/vetworld.2022.2903-2909

Copyright: Srichumporn, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.