A urinary proteomic study in hypercalciuric dogs with and without calcium oxalate urolithiasis

Vet World Vol.15 December-2022 Article-24

Research Article

Veterinary World, 15(12): 2937-2944

https://doi.org/10.14202/vetworld.2022.2937-2944

Sumonwan Chamsuwan¹, Chollada Buranakarl¹, Kris Angkanaporn¹, Thasinas Dissayabutra², Natthaya Chuaypen², Trairak Pisitkun³, and Nuttiya Kalpongnukul³

1. Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
2. Metabolic Disease in Gut and Urinary System Research Unit, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
3. Center of Excellence in Systems Biology, Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Background and Aim: Hypercalciuria is an important predisposing factor commonly found in humans and dogs with calcium oxalate (CaOx) urolithiasis. Calcium oxalate crystals can induce an inflammatory reaction that subsequently produces several proteins that have an inhibitory or stimulatory effect on stone formation. This study aimed to evaluate the differences in urinary proteomic profiles between hypercalciuric CaOx stone dogs and hypercalciuric stone-free dogs (CaOx stone and control groups, respectively).

Materials and Methods: Seven dogs with hypercalciuric CaOx urolithiasis and breed-, sex-, and aged-matched controls with hypercalciuria were included in the study. Serum and urine samples were obtained from all dogs to analyze electrolytes. Urinary proteomic profiles were analyzed using liquid chromatography-mass spectrometry. Student's t-test was used to compare the differences between groups.

Results: Forty-nine urinary proteins were identified in the stone-free and CaOx stone groups, whereas 19 and 6 proteins were unique in the CaOx stone and stone-free groups, respectively. The urinary thrombomodulin level was significantly higher in the CaOx stone group (relative ratio = 1.8, p < 0.01) than in the stone-free group.

Conclusion: This study demonstrated that urinary proteomic profiles may be used as a candidate biomarker for urinary tract injury in CaOx urolithiasis in dogs. Keywords: calcium oxalate stone, dog, hypercalciuria, urinary biomarker.

Keywords: calcium oxalate stone, dog, hypercalciuria, urinary biomarker.

How to cite this article: Chamsuwan S, Buranakarl C, Angkanaporn K, Dissayabutra T, Chuaypen N, Pisitkun T, and Kalpongnukul N (2022) A urinary proteomic study in hypercalciuric dogs with and without calcium oxalate urolithiasis, Veterinary World, 15(12): 2937–2944.

Received: 08-08-2022 Accepted: 04-11-2022 Published online: 27-12-2022

Corresponding author: Chollada Buranakarl E-mail: bchollad@chula.ac.th

DOI: 10.14202/vetworld.2022.2937-2944

Copyright: Chamsuwan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.