Molecular characterization of Newcastle disease virus vaccines in Nigeria

Vet World Vol.15 December-2022 Article-8

Research Article

Veterinary World, 15(12): 2816-2821

https://doi.org/10.14202/vetworld.2022.2816-2821

Mohammed Usman Sajo^1,2,3, Lawal Sa'idu^1,4, Maman Moutari Souley⁵, and Olusegun Adesina Fagbohun^1,2

1. Institute of Life and Earth Sciences, Pan African University, (PAULESI), Ibadan, Nigeria.
2. Department of Veterinary Microbiology, University of Ibadan, Ibadan, Nigeria.
3. Department of Veterinary Microbiology, University of Maiduguri, Maiduguri, Nigeria.
4. Veterinary Teaching Hospital, Ahmadu Bello University, Zaria, Nigeria.
5. Laboratoire Central de L'Elevage, B.P: 485, Niamey, Niger Republic.

Background and Aim: Newcastle disease (ND) caused by ND virus (NDV) is a serious impediment to effective poultry production in developing countries such as Nigeria. Despite employing vaccination and other control measures to curtail this disease, its severe forms still persist. This study aimed to confirm the virus strains in the NDV vaccine brands commonly used in Nigeria.

Materials and Methods: We employed reverse-transcription polymerase chain reaction (RT-PCR), sequencing, and sequence analysis to characterize NDV strains in four NDV vaccines commonly used in Nigeria. Fragments of 300 bp from NDV fusion genes from the vaccines were amplified. Polymerase chain reaction products were sequenced and analyzed using multiple sequence alignment and phylogenetic analyses to characterize the vaccine viruses as pathotypes.

Results: All the vaccines gave positive results, confirming the presence of NDV. Multiple sequence alignment and phylogenetic analyses revealed that two of the vaccines had the lentogenic pathotype, while the other two had the mesogenic or velogenic pathotype.

Conclusion: This study provides information to facilitate strategies for regular control of the quality of vaccines in Nigeria. Keywords: Newcastle disease virus, Nigeria, polymerase chain reaction, vaccines.

Keywords: Newcastle disease virus, Nigeria, polymerase chain reaction, vaccines.

How to cite this article: Sajo MU, Sa'idu L, Souley MM, and Fagbohun OA (2022) Molecular characterization of Newcastle disease virus vaccines in Nigeria, Veterinary World, 15(12): 2816–2821.

Received: 23-07-2022 Accepted: 27-10-2022 Published online: 10-12-2022

Corresponding author: Olusegun Adesina Fagbohun E-mail: oa.fagbohun@ui.edu.ng

DOI: 10.14202/vetworld.2022.2816-2821

Copyright: Sajo, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.