Cervical anticancer activities of Annona squamosa Linn. leaf isolate

Vet World Vol.15 January-2022 Article-16

Research Article

Veterinary World, 15(1): 124-131

https://doi.org/10.14202/vetworld.2022.124-131

Made Dira Swantara¹, Wiwik Susanah Rita¹, Made Asmarani Dira², and Kadek Karang Agustina³

1. Department of Applied Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, Denpasar, Bali 80225 Indonesia.
2. Clinical and Community Pharmacy Study Program, Faculty of Health, Bali Institute of Technology and Health, Denpasar, Bali 80225, Indonesia.
3. Department of Public Health, The Faculty of Veterinary Medicine, Udayana University, Denpasar, Bali 80225, Indonesia.

Background and Aim: Cancer is one of the leading causes of death, the need for new anticancer herbal drugs is becoming more urgent considering the side effects of synthetic drugs. This study aimed to determine the anticancer activity of isolates derived from the methanol extract of Annona squamosa Linn. leaves and to identify the compounds that have an active effect against HeLa cells.

Materials and Methods: The leaf metabolites of A. squamosa L. were extracted using methanol at room temperature (28°C) and were partitioned into n-hexane, chloroform, and n-butanol. The toxicity test of these extracts was conducted using a brine shrimp lethality assay. Furthermore, the most toxic extracts were separated and purified using silica gel column chromatography to yield four isolate fractions: FA, FB, FC, and FD. The most toxic isolates were tested for anticancer against HeLa cells, and their compounds were identified using liquid chromatography-mass spectrometry.

Results: The results showed that the most toxic isolate with an LC50 value of 100.00 ppm had a potency similar to that of an anticancer agent with an IC50 value of 70.9021 ppm. Furthermore, the five compounds identified in this isolate include (6S, 7aR)-6-hydroxy-4,4,7a-trimethyl-6,7-dihydro-5H-1-benzofuran-2-one or loliolide, cocamidopropyl betaine, N-[3- (dimethylamino)propyl]dodecanamide or lauramidopropyl dimethylamine, linolenic acid, and 1-dodecyl-2-azepanone or laurocapram.

Conclusion: It can be concluded that the leaf isolates of A. squamosa Linn. had shown anticancer activities against cervical cancer. Keywords: Annona squamosa Linn, cervical anticancer activities, HeLa cells.

Keywords: Annona squamosa Linn, cervical anticancer activities, HeLa cells.

How to cite this article: Swantara MD, Rita WS, Dira MA, Agustina KK (2022) Cervical anticancer activities of Annona squamosa Linn. leaf isolate, Veterinary World, 15(1): 124-131.

Received: 16-09-2021 Accepted: 20-12-2021 Published online: 24-01-2022

Corresponding author: E-mail: dira_swantara@unud.ac.id

DOI: 10.14202/vetworld.2022.124-131

Copyright: Swantara, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.