Vet World Vol.15 January-2022 Article-28
Research Article
Veterinary World, 15(1): 220-231
https://doi.org/10.14202/vetworld.2022.220-231
Prospects of bacteriophage collections in disinfectant applications
2. Laboratory of Microbiology, Research Institute for Biological Safety Problems, Gvardeiskiy Urban-type village, Kordaiskiy region, Zhambylskaya Oblast, Republic of Kazakhstan.
3. Department of Clinical Veterinary Medicine, Kazakh National Agrarian Research University, Almaty, Republic of Kazakhstan.
Background and Aim: The search and development of disinfectants is promising worldwide. However, there are currently no international regulations governing the testing and registration of germicidal agents. Moreover, the number of safety requirements for disinfectants for human, animal, and environmental health has increased. This research aimed to evaluate the prospects of using a collection of bacteriophages for disinfectant purposes.
Materials and Methods: The objects of research were bacteriophages isolated from a total of 129 environmental samples obtained from seven sources in and around livestock buildings: (1) Feed residues from feeders and automatic drinkers; (2) washouts from floors, walls, and posts; (3) soil from underneath floors; (4) bedding; (5) sewage; (6) ponds; and (7) soil from paddocks. The corresponding strains were used as indicator test cultures for bacteriophages. The authors employed the following methods to work with bacteriophages: (a) Bacteriophage isolation methods, (b) the Appelman method (i.e., serial dilutions), (c) the Grazia method (i.e., agar layers), (d) phage titration on solid media, and (e) the bacterial phagotyping method.
Results: The results of the analysis on the bacteria of the Enterobacteriaceae family isolated 11 bacteriophages; one bacteriophage is specific to Pseudomonas aeruginosa, and another one is specific to Brucella abortus. The results also indicate that all bacteriophage strains of the Enterobacteriaceae family demonstrate lysis at a pH of 7.0. In addition, this polyphage lyses all strains of sensitive bacterial cultures. The optimum temperature for the cultivation of bacteriophages is 35°C. While using electron microscopy to study the consortium of bacteriophages, clearly distinguishable virions of bacteriophages were found in the microscope field of view.
Conclusion: The main parameters for the production of polyphages include the ratio of the bacteriophage and its corresponding bacteriophage-sensitive culture, the pH of the cultivation medium, and the cultivation time of the bacteriophage system as well as the sensitive bacterium. With regard to the aforementioned parameters, the results indicate that the average value for all bacteriophages is 1:2, and the average cultivation medium pH is 7.0 for all bacteriophages. The average cultivation time for all bacteriophages is 18-24 h. Keywords: bacteriophages, biological preparation, disinfection, lytic activity, strain, titer.
Keywords: bacteriophages, biological preparation, disinfection, lytic activity, strain, titer.
How to cite this article: Issabekov SS, Syrym NS, Sambetbayev AA, Alikhanov KD, Yespembetov BA (2022) Prospects of bacteriophage collections in disinfectant applications, Veterinary World, 15(1): 220-231.
Received: 24-06-2021 Accepted: 25-11-2021 Published online: 31-01-2022
Corresponding author: E-mail: s.issabekov@bk.ru
DOI: 10.14202/vetworld.2022.220-231
Copyright: Issabekov, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.