Vet World   Vol.15   July-2022  Article-12

Research Article

Veterinary World, 15(7): 1691-1698

https://doi.org/10.14202/vetworld.2022.1691-1698

Determination of multidrug-resistant populations and molecular characterization of complex Klebsiella spp. in wild animals by multilocus sequence typing

Alessandra Tammy Hayakawa Ito de Sousa1, Marco Túlio dos Santos Costa1, Stefhano Luis Cândido1, Herica Makino1, Thais Oliveira Morgado2, Lucas Avelino Dandolini Pavelegini3, Edson Moleta Colodel3, Luciano Nakazato1, and Valéria Dutra1
1. Microbiology Laboratory of the Veterinary Hospital, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
2. Center for Medicine and Research of Wild Animals, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
3. Pathology Laboratory of the Veterinary Hospital, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.

Background and Aim: One of the most significant public health concerns is multidrug-resistant (MDR) microorganisms. Klebsiella spp. have been at the forefront of causing different types of infections such as bacteremia, urinary tract infections, pneumonia, enteritis, and sepsis in humans as well as animals. This study aimed to determine the genomic similarity between Klebsiella spp. isolated from wild animal samples and those described in the Institut Pasteur genomic database to verify the spread of resistant clones regionally in the state of Mato Grosso, and to compare the epidemiological data in different regions of Brazil and the world.

Materials and Methods: Isolates from various sites of injury in wild animals were identified by sequencing the 16S rRNA gene. Antimicrobial susceptibility testing was performed using the disk diffusion method to verify the resistance profile, and then, multilocus sequence typing was performed to verify the population structure and compare the isolates from other regions of Brazil and the world.

Results: Twenty-three sequence types (STs) were observed; of these, 11 were new STs, as new alleles were detected. There was no predominant ST among the isolates. All isolates were MDR, with high rates of resistance to sulfonamides, ampicillin, amoxicillin, and nitrofurantoin and low resistance to meropenem, imipenem, and amikacin.

Conclusion: Improving our understanding of the population structure of Klebsiella spp. in wild animals may help determine the source of infection during outbreaks in humans or animals, as the One Health concept emphasizes the interlinks between humans, animals, and environmental health. Keywords: antimicrobial, Klebsiella, multidrug resistance, multilocus sequence typing, susceptibility, wild animals.

Keywords: antimicrobial, Klebsiella, multidrug resistance, multilocus sequence typing, susceptibility, wild animals.

How to cite this article: de Sousa ATHI, Costa MTS, Cândido SL, Makino H, Morgado TO, Pavelegini LAD, Colodel EM, Nakazato L, Dutra V (2022) Determination of multidrug-resistant populations and molecular characterization of complex Klebsiella spp. in wild animals by multilocus sequence typing, Veterinary World, 15(7): 1691–1698.

Received: 01-02-2022  Accepted: 26-05-2022     Published online: 19-07-2022

Corresponding author: Valéria Dutra   E-mail: valeriadutra.dutra@gmail.com

DOI: 10.14202/vetworld.2022.1691-1698

Copyright: de Sousa ATHI, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.