Vet World   Vol.15   July-2022  Article-19

Silver nanoparticle-conjugated antibiotics inhibit in vitro growth of Brucella melitensis

Background and Aim: Brucellosis is a contagious livestock disease with a significant economic impact. This study aimed to compare the efficacy of antibiotics used alone or in combination with silver nanoparticles (AgNPs) against Brucella melitensis Rev 1 in vitro.

Materials and Methods: AgNps conjugated with ciprofloxacin was synthesized and thoroughly characterized by ultraviolet visible spectrophotometry (UV-vis). The antimicrobial effect of ciprofloxacin alone and ciprofloxacin conjugated with AgNPs against B. melitensis Rev 1 was determined by minimum inhibitory concentration (MIC) and the erythrocyte hemolytic assay determined the capability of conjugation to cause hemolysis in human erythrocyte.

Results: The UV-vis spectra of both silver-drug nanoconjugates showed a characteristic surface plasmon resonance band at 420 nm. The MIC assays showed that AgNPs conjugation to antibiotics enhanced the antibacterial potential of the selected antibiotics against B. melitensis Rev 1 relative to non-conjugated antibiotics. The results show that low concentrations of AgNPs can kill B. melitensis Rev 1. The MICs of ciprofloxacin and ciprofloxacin–AgNPs were 0.75 and 0.05 μM, respectively.

Conclusion: The conjugation of ciprofloxacin with AgNPs enhanced the antibacterial effects against B. melitensis Rev 1. In addition, this conjugation appears to inhibit the capability of this bacterium to adapt to the presence of antibiotics, thereby inhibiting bacterial resistance. Further studies are required to examine its potential as an in vivo treatment. Keywords: antimicrobial activity, Brucella melitensis Rev 1, minimum inhibitory concentrations, silver nanoparticles.

Keywords: antimicrobial activity, Brucella melitensis Rev 1, minimum inhibitory concentrations, silver nanoparticles.

How to cite this article: Saadh MJ (2022) Silver nanoparticle-conjugated antibiotics inhibit in vitro growth of Brucella melitensis, Veterinary World, 15(7): 1749–1752.

Received: 02-03-2022  Accepted: 03-06-2022     Published online: 22-07-2022

Corresponding author: Mohamed J. Saadh   E-mail: msaadeh@meu.edu.jo

DOI: 10.14202/vetworld.2022.1749-1752

Copyright: Saadh, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.