Vet World Vol.15 July-2022 Article-7
Research Article
Veterinary World, 15(7): 1650-1656
https://doi.org/10.14202/vetworld.2022.1650-1656
Identification of cellulolytic lactic acid bacteria from the intestines of laying hens given AKBISprob based on 16S ribosomal ribonucleic acid gene analysis
2. Study Program of Veterinary Education, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia.
3. Laboratory of Research, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia.
4. Laboratory of Biochemical, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia.
5. Laboratory of Clinic, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia.
Background and Aim: Supplementation of AKBISprob (developed in a previous study) in feed can improve production efficiency and poultry health, especially laying hens. In addition, it can also increase cellulolytic lactic acid bacteria (LAB) in chicken intestines, but these bacteria are still unknown; thus, they need to be identified. This study aimed to identify cellulolytic LAB in the intestines of laying hens administered AKBISprob based on 16S ribosomal ribonucleic acid (16S rRNA) gene analysis.
Materials and Methods: The samples used in this study were 13 LAB isolates from the intestines of laying hens that were given AKBISprob 4%. Cellulolytic LAB DNA was isolated and 16S rRNA gene was amplified by polymerase chain reaction, followed by sequencing, bioinformatics analysis, and phylogenetic tree construction.
Results: From 10 cellulolytic LAB isolates with a clear zone of >6 mm, four were selected and their DNA was amplified with BaCF and UniB primers ∼1500 bp DNA fragments. Of these, the P31H62 isolate was genetically close to Enterococcus hirae strain 1-1X-16 with 92.90% maximum identity, the P33S52 isolate had homology with Enterococcus mundtii strain ZU 26 with 96.76% maximum identity, and the P33S62 isolate was closely related to E. hirae strain SJ3 with 72.96% maximum identity. The phylogenetic tree revealed that the cellulolytic LAB isolates P31H62 and P33S52 were in one cluster closely related to the genus Enterococcus.
Conclusion: This study suggests that the isolates P31H62, P33S62, and P33S52 from the intestines of laying hens administered 4% AKBISprob are cellulolytic LAB belonging to the genus Enterococcus. Keywords: 16S ribosomal ribonucleic acid, AKBISprob, Enterococcus, phylogenetic tree, polymerase chain reaction.
Keywords: 16S ribosomal ribonucleic acid, AKBISprob, Enterococcus, phylogenetic tree, polymerase chain reaction.
How to cite this article: Nurliana N, Siregar BH, Sari WE, Helmi TZ, Sugito S (2022) Identification of cellulolytic lactic acid bacteria from the intestines of laying hens given AKBISprob based on 16S ribosomal ribonucleic acid gene analysis, Veterinary World, 15(7): 1650–1656.
Received: 07-12-2021 Accepted: 12-05-2022 Published online: 14-07-2022
Corresponding author: Nurliana Nurliana E-mail: nurliana.nuna@unsyiah.ac.id
DOI: 10.14202/vetworld.2022.1650-1656
Copyright: Nurliana, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.